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Literature summary for 3.2.1.3 extracted from

  • De Mot, R.; van Oudendijck, E.; Verachtert, H.
    Purification and characterization of an extracellular glucoamylase from the yeast Candida tsukubaensis CBS 6389 (1985), Antonie van Leeuwenhoek, 51, 275-287.
    View publication on PubMed
Search for more literature for 3.2.1.3

Inhibitors

Inhibitors Comment Organism Structure
acarbose 25 ng/ml, 50% inhibition Pseudozyma tsukubaensis
alpha-cyclodextrins 10 mM, 3% inhibition Pseudozyma tsukubaensis
beta-cyclodextrins 10 mM, 48% inhibition Pseudozyma tsukubaensis
Ca2+
-
 Pseudozyma tsukubaensis
EDTA 10 mM, 16% inhibition Pseudozyma tsukubaensis
methyl alpha-D-glucoside 10 mM, 30% inhibition Pseudozyma tsukubaensis

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Pseudozyma tsukubaensis
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
56000
-
 x * 56000, SDS-PAGE Pseudozyma tsukubaensis

Organism

Organism UniProt Comment Textmining
Pseudozyma tsukubaensis
-
 CBS 6389
-
Pseudozyma tsukubaensis CBS 6389
-
 CBS 6389
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Pseudozyma tsukubaensis

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudozyma tsukubaensis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5507
-
-
 Pseudozyma tsukubaensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl alpha-D-glucoside + H2O 6% of the activity with maltose Pseudozyma tsukubaensis 4-nitrophenol + D-glucose
-
 ?
alpha-cyclodextrin + H2O 6% of the activity with maltose Pseudozyma tsukubaensis glucose + ?
-
 ?
amylopectin + H2O
-
 Pseudozyma tsukubaensis D-glucose + ?
-
 ?
amylopectin + H2O
-
 Pseudozyma tsukubaensis CBS 6389 D-glucose + ?
-
 ?
amylose + H2O
-
 Pseudozyma tsukubaensis glucose + ?
-
 ?
amylose + H2O
-
 Pseudozyma tsukubaensis CBS 6389 glucose + ?
-
 ?
beta-cyclodextrin + H2O 6% of the activity with maltose Pseudozyma tsukubaensis ?
-
 ?
dextrin + H2O
-
 Pseudozyma tsukubaensis ?
-
 ?
dextrin + H2O
-
 Pseudozyma tsukubaensis CBS 6389 ?
-
 ?
glycogen + H2O
-
 Pseudozyma tsukubaensis glucose + ?
-
 ?
glycogen + H2O
-
 Pseudozyma tsukubaensis CBS 6389 glucose + ?
-
 ?
maltose + H2O
-
 Pseudozyma tsukubaensis 2 glucose
-
 ?
maltose + H2O
-
 Pseudozyma tsukubaensis CBS 6389 2 glucose
-
 ?
maltotriose + H2O
-
 Pseudozyma tsukubaensis maltose + glucose
-
 ?
methyl-alpha-D-glucoside + H2O 5% of the activity with maltose Pseudozyma tsukubaensis methanol + alpha-D-glucose
-
 ?
pullulan + H2O
-
 Pseudozyma tsukubaensis ?
-
 ?
sucrose + H2O
-
 Pseudozyma tsukubaensis D-glucose + D-fructose
-
 ?

Subunits

Subunits Comment Organism
? x * 56000, SDS-PAGE Pseudozyma tsukubaensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 Pseudozyma tsukubaensis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 30 min, stable Pseudozyma tsukubaensis
50
-
 30 min, 85% loss of activity Pseudozyma tsukubaensis
60
-
 5 min, more than 95% loss of activity Pseudozyma tsukubaensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4 4.8
-
 Pseudozyma tsukubaensis

pH Stability

pH Stability pH Stability Maximum Comment Organism
5
-
 quite stable below Pseudozyma tsukubaensis