Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Sulfolobus acidocaldarius |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
63600 | - |
recombinant enzyme, SDS-PAGE | Sulfolobus acidocaldarius |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sulfolobus acidocaldarius | A0A2Z5U3T3 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Sulfolobus acidocaldarius |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha,alpha-trehalose + H2O | - |
Sulfolobus acidocaldarius | beta-D-glucose + alpha-D-glucose | - |
? | |
additional information | the enzyme does not hydrolyze any other trehalose analogs such as maltose, nigerose, and turanose | Sulfolobus acidocaldarius | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Saci_1816 | locus name | Sulfolobus acidocaldarius |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
recombinant enzyme | Sulfolobus acidocaldarius |
80 | 85 | native enzyme | Sulfolobus acidocaldarius |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 75 | 40°C: about 75% of maximal activity, 75°C: about 35% of maximal activity, recombinant enzyme | Sulfolobus acidocaldarius |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
thermostability of recombinant enzyme is slightly reduced compared to native enzyme | Sulfolobus acidocaldarius |
90 | - |
half-life of native enzyme: 53 min, half-life of revcombinant enzyme: 41 min | Sulfolobus acidocaldarius |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | - |
recombinant enzyme | Sulfolobus acidocaldarius |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 5 | pH 3.0: about 50% of maximal activity, pH 5.0: about 40% of maximal activity, recombinant enzyme | Sulfolobus acidocaldarius |