Cloned (Comment) | Organism |
---|---|
expressed in Pichia pastoris strain KM71 | Metarhizium anisopliae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
trehazolin | specific inhibitor | Metarhizium anisopliae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.3 | - |
trehalose | native enzyme, in 20 mM MES, pH 6.0 for 10 min at 30°C | Metarhizium anisopliae | |
2.6 | - |
trehalose | recombinant enzyme, in 20 mM MES, pH 6.0 for 10 min at 30°C | Metarhizium anisopliae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
170000 | - |
SDS-PAGE, recombinant enzyme | Metarhizium anisopliae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Metarhizium anisopliae | A9XE63 | fragment | - |
Purification (Comment) | Organism |
---|---|
Ni-NTA affinity column chromatography | Metarhizium anisopliae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
101.3 | - |
recombinant enzyme, after purification, at 30°C | Metarhizium anisopliae |
120 | - |
native enzyme, after purification, at 30°C | Metarhizium anisopliae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
trehalose + H2O | high specificity for trehalose | Metarhizium anisopliae | D-glucose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
acid trehalase | - |
Metarhizium anisopliae |
ATM1 | mature enzyme | Metarhizium anisopliae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
recombinant enzyme | Metarhizium anisopliae |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
15 | 50 | recombinant enzyme | Metarhizium anisopliae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
the enzyme is stable below 50°C, but it loses activity rapidly above 50°C | Metarhizium anisopliae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
recombinant enzyme | Metarhizium anisopliae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | 7.5 | recombinant enzyme | Metarhizium anisopliae |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3.5 | 8 | after incubation at pH values of 3.5-8.0 and 30°C for 1 h, all residual activities of reATM1 are over 80% | Metarhizium anisopliae |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Metarhizium anisopliae | native enzyme, isoelectric focusing | - |
4.7 |
Metarhizium anisopliae | recombinant enzyme, isoelectric focusing | - |
4.9 |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.000013 | - |
native enzyme, in 20 mM MES, pH 6.0, at 30°C | Metarhizium anisopliae | trehazolin | |
0.000019 | - |
recombinant enzyme, in 20 mM MES, pH 6.0, at 30°C | Metarhizium anisopliae | trehazolin |