Activating Compound | Comment | Organism | Structure |
---|---|---|---|
alpha,alpha-trehalose | the enzymatic activity increases with increasing concentrations of alpha,alpha-trehalose in the incubations up to about 50 mM | Mycolicibacterium smegmatis | |
arsenate | - |
Mycolicibacterium smegmatis | |
phosphate | - |
Mycolicibacterium smegmatis |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain BL21 | Mycolicibacterium smegmatis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AMP | 50% inhibition at 20 mM | Mycolicibacterium smegmatis | |
ATP | 90% inhibition at 20 mM | Mycolicibacterium smegmatis | |
castanospermine | 50% inhibition at 0.5 mg/ml | Mycolicibacterium smegmatis | |
diphosphate | more than 50% inhibition at 8 mM and complete inhibition at 16 mM | Mycolicibacterium smegmatis | |
additional information | 40 mM sodium fluoride and trehazolin have no inhibitory effect | Mycolicibacterium smegmatis | |
Polyphosphate | - |
Mycolicibacterium smegmatis | |
sodium orthovanadate | 80% inhibition at 10 mM | Mycolicibacterium smegmatis | |
UTP | 90% inhibition at 10 mM | Mycolicibacterium smegmatis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
20 | - |
alpha,alpha-trehalose | at 37°C | Mycolicibacterium smegmatis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Mycolicibacterium smegmatis | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | absolutely required for activity, optimum activity occurring at concentrations of 3.5-4 mM | Mycolicibacterium smegmatis | |
additional information | Mg2+ can not be replaced by Ca2+, Mn2+ or Zn2+ | Mycolicibacterium smegmatis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
71000 | - |
SDS-PAGE | Mycolicibacterium smegmatis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycolicibacterium smegmatis | - |
- |
- |
Mycolicibacterium smegmatis b11 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate fractionation, phenyl-Sepharose DE-52 column chromatography and Sephacryl S-300 gel filtration | Mycolicibacterium smegmatis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.227 | - |
cell extract, at 37°C | Mycolicibacterium smegmatis |
36.99 | - |
after 162fold purification, at 37°C | Mycolicibacterium smegmatis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha,alpha-trehalose + H2O | highly specific for alpha,alpha-trehalose | Mycolicibacterium smegmatis | 2 D-glucose | - |
? | |
alpha,alpha-trehalose + H2O | highly specific for alpha,alpha-trehalose | Mycolicibacterium smegmatis b11 | 2 D-glucose | - |
? | |
additional information | does not hydrolyze alpha,beta-trehalose, beta,beta-trehalose, trehalose dimycolate, or any other alpha-glucoside or beta-glucoside | Mycolicibacterium smegmatis | ? | - |
? | |
additional information | does not hydrolyze alpha,beta-trehalose, beta,beta-trehalose, trehalose dimycolate, or any other alpha-glucoside or beta-glucoside | Mycolicibacterium smegmatis b11 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
multimer | 20 or more subunits, gel filtration | Mycolicibacterium smegmatis |
Synonyms | Comment | Organism |
---|---|---|
MSMEG 4528 | - |
Mycolicibacterium smegmatis |
trehalase | - |
Mycolicibacterium smegmatis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.1 | - |
- |
Mycolicibacterium smegmatis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8 | the trehalase activity drops sharply at pH values of 6.5 and below, as well as at pH values of 8.0 and above | Mycolicibacterium smegmatis |