Cloned (Comment) | Organism |
---|---|
expressed in Pichia pastoris as a His-tagged fusion protein | Aspergillus niger |
Protein Variants | Comment | Organism |
---|---|---|
S259F/P261T/L262S/G263S | mutant shows slightly reduced affinity for substrate (Km: 3.76 mM), 8.5fold reduction in inhibition by galactose, maximal activity lower than wild-type, pH stability lower than wild-type | Aspergillus niger |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-galactose | - |
Aspergillus niger |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.21 | - |
2-nitrophenyl beta-D-galactopyranoside | wild-type, pH 5.5, 42° | Aspergillus niger | |
3.76 | - |
2-nitrophenyl beta-D-galactopyranoside | mutant S259F/P261T/L262S/G263S, pH 5.5, 42° | Aspergillus niger |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus niger | B6HYI9 | - |
- |
Purification (Comment) | Organism |
---|---|
using Ni-NTA chromatography | Aspergillus niger |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-nitrophenyl beta-D-galactopyranoside + H2O | - |
Aspergillus niger | 2-nitrophenol + beta-D-galactose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
beta-galactosidase | - |
Aspergillus niger |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
2.5 | - |
wild-type | Aspergillus niger |
4.5 | - |
mutant S259F/P261T/L262S/G263S | Aspergillus niger |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.76 | - |
D-galactose | wild-type, pH 5.5, 42° | Aspergillus niger | |
6.46 | - |
D-galactose | mutant S259F/P261T/L262S/G263S, pH 5.5, 42° | Aspergillus niger |