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Literature summary for 3.2.1.23 extracted from

  • Crescimbeni, M.C.; Nolan, V.; Clop, P.D.; Marin, G.N.; Perillo, M.A.
    Activity modulation and reusability of beta-D-galactosidase confined in sol-gel derived porous silicate glass (2010), Colloids Surf. B Biointerfaces, 76, 387-396.
    View publication on PubMed

General Stability

General Stability Organism
about 40% of the initial enzymatic activity is recovered after beta-Gal encapsulation in the silica matrix Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
NaCl the activity of both soluble beta-Gal and encapsulated beta-Gal decrease with salt addition. While in the case of soluble beta-Gal at 150 mM NaCl the reaction rate has decreased to half of the control value, in the case of encapsulated bet-Gal the same inhibition level is reached at 300 mM NaCl Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0836
-
2-nitrophenyl beta-D-galactopyranoside enzyme encapsulated in a silica matrix Escherichia coli
0.122
-
2-nitrophenyl beta-D-galactopyranoside soluble enzyme Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
lactose + H2O Escherichia coli
-
D-galactose + D-glucose
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-nitrophenyl beta-D-galactopyranoside + H2O
-
Escherichia coli 2-nitrophenol + beta-D-galactose
-
?
lactose + H2O
-
Escherichia coli D-galactose + D-glucose
-
?

Synonyms

Synonyms Comment Organism
beta-D-galactosidase
-
Escherichia coli
beta-gal
-
Escherichia coli

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37 75 soluble bet-Gal is completely inactivated when it is pre-incubated at 75°C, whereas encapsulated bet-Gal retains above 90% of its maximum activity, even after being reused, preincubation at 37°C does not affect the enzymatic activity Escherichia coli

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 10 the enzymatic activity of encapsulated beta-Gal remains constant after pre-incubation at pH 4-10. However, while at pH 2 the soluble enzyme is completely inactivated, encapsulated beta-Gal retains 40% of its maximum activity Escherichia coli