General Stability | Organism |
---|---|
about 40% of the initial enzymatic activity is recovered after beta-Gal encapsulation in the silica matrix | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NaCl | the activity of both soluble beta-Gal and encapsulated beta-Gal decrease with salt addition. While in the case of soluble beta-Gal at 150 mM NaCl the reaction rate has decreased to half of the control value, in the case of encapsulated bet-Gal the same inhibition level is reached at 300 mM NaCl | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0836 | - |
2-nitrophenyl beta-D-galactopyranoside | enzyme encapsulated in a silica matrix | Escherichia coli | |
0.122 | - |
2-nitrophenyl beta-D-galactopyranoside | soluble enzyme | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
lactose + H2O | Escherichia coli | - |
D-galactose + D-glucose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-nitrophenyl beta-D-galactopyranoside + H2O | - |
Escherichia coli | 2-nitrophenol + beta-D-galactose | - |
? | |
lactose + H2O | - |
Escherichia coli | D-galactose + D-glucose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
beta-D-galactosidase | - |
Escherichia coli |
beta-gal | - |
Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | 75 | soluble bet-Gal is completely inactivated when it is pre-incubated at 75°C, whereas encapsulated bet-Gal retains above 90% of its maximum activity, even after being reused, preincubation at 37°C does not affect the enzymatic activity | Escherichia coli |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 10 | the enzymatic activity of encapsulated beta-Gal remains constant after pre-incubation at pH 4-10. However, while at pH 2 the soluble enzyme is completely inactivated, encapsulated beta-Gal retains 40% of its maximum activity | Escherichia coli |