Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 53% rise in activity in the presence of 10 mM 2-beta-mercaptoethanol | Arthrobacter sp. | |
dithiothreitol | 25% rise in activity in the presence of 10 mM dithiothreitol | Arthrobacter sp. | |
additional information | the enzyme is not stimulated by glutathione | Arthrobacter sp. |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli TOP10F cells | Arthrobacter sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-chloromercuribenzoic acid | complete inhibition at 10 mM | Arthrobacter sp. | |
Ca2+ | 95% residual activity at 5 mM | Arthrobacter sp. | |
Cu2+ | complete inhibition at 5 mM | Arthrobacter sp. | |
Pb2+ | 15% residual activity at 5 mM | Arthrobacter sp. | |
Zn2+ | 50% residual activity at 5 mM | Arthrobacter sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | 49% increase of activity at 5 mM | Arthrobacter sp. | |
Mn2+ | 11% increase of activity at 5 mM | Arthrobacter sp. | |
additional information | the enzyme is not affected by Mg2+ | Arthrobacter sp. | |
Na+ | 53% increase of activity at 5 mM | Arthrobacter sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
113695 | - |
4 * 113695, calculated from amino acid sequence | Arthrobacter sp. |
116000 | - |
4 * 116000, native and SDS-PAGE | Arthrobacter sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
lactose + H2O | Arthrobacter sp. | - |
D-galactose + D-glucose | - |
? | |
lactose + H2O | Arthrobacter sp. 20B | - |
D-galactose + D-glucose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arthrobacter sp. | - |
- |
- |
Arthrobacter sp. 20B | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DEAE-Sepharose column chromatography, PABTG-Sepharose column chromatography, and Sephacryl S-300 gel filtration | Arthrobacter sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.35 | - |
cell extract, in 50 mM potassium phosphate buffer, pH 7.3, containing 100 mM NaCl and 100 mM beta-mercaptoethanol | Arthrobacter sp. |
2.6 | - |
after 7fold purification, in 50 mM potassium phosphate buffer, pH 7.3, containing 100 mM NaCl and 100 mM beta-mercaptoethanol | Arthrobacter sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-nitrophenyl beta-D-galactopyranoside + H2O | 3times lower activity than for 4-nitrophenyl-beta-D-galactopyranoside | Arthrobacter sp. | 2-nitrophenol + beta-D-galactose | - |
? | |
2-nitrophenyl beta-D-galactopyranoside + H2O | 3times lower activity than for 4-nitrophenyl-beta-D-galactopyranoside | Arthrobacter sp. 20B | 2-nitrophenol + beta-D-galactose | - |
? | |
4-nitrophenyl beta-D-galactopyranoside + H2O | preferred substrate, 3times higher activity than for 2-nitrophenyl-beta-D-galactopyranoside | Arthrobacter sp. | 4-nitrophenol + beta-D-galactose | - |
? | |
4-nitrophenyl beta-D-galactopyranoside + H2O | preferred substrate, 3times higher activity than for 2-nitrophenyl-beta-D-galactopyranoside | Arthrobacter sp. 20B | 4-nitrophenol + beta-D-galactose | - |
? | |
lactose + H2O | - |
Arthrobacter sp. | D-galactose + D-glucose | - |
? | |
lactose + H2O | - |
Arthrobacter sp. 20B | D-galactose + D-glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 113695, calculated from amino acid sequence | Arthrobacter sp. |
homotetramer | 4 * 116000, native and SDS-PAGE | Arthrobacter sp. |
Synonyms | Comment | Organism |
---|---|---|
BgaS | - |
Arthrobacter sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
- |
Arthrobacter sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
- |
45 | the enzyme displays 52-85% maximum activity at 0-20°C, about 50% activity is seen at 45°C | Arthrobacter sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
- |
35 | the enzyme remains stable from 0-35°C, above 35°C there is a constant drop of activity with about 60% relative activity at 40°C, 30% relative activity at 45°C, and no activity at 60-70°C | Arthrobacter sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | - |
Arthrobacter sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | - |
Arthrobacter sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | 8 | the enzyme remains stable from pH 5.5-8.0, about 40% relative activity at pH 4.0 and 10.0, about 70% relative activity at pH 5.0 and 9.0, less than 10% activity at pH 11.0 | Arthrobacter sp. |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
5 | - |
at 25°C | Arthrobacter sp. | Zn2+ |