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Literature summary for 3.2.1.23 extracted from
- Effects of galactose and glucose on the hydrolysis reaction of a thermostable beta-galactosidase from Caldicellulosiruptor saccharolyticus (2010), Appl. Microbiol. Biotechnol., 85, 1427-1435.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli ER2566 cells | Caldicellulosiruptor saccharolyticus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| D-galactose | uncompetitive inhibition, 26% residual activity at 50 g/l, when adding D-galactose (200 g/l) only 14% of the lactose s hydrolyzed after 180 min | Caldicellulosiruptor saccharolyticus |  |
| D-glucose | 82% residual activity at 400 g/l | Caldicellulosiruptor saccharolyticus | |
| additional information | addition of D-glucose (400 g/l) does not affect lactose hydrolysis | Caldicellulosiruptor saccharolyticus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.11 | - |
4-nitrophenyl beta-D-galactopyranoside | at pH 6.0 and 80°C | Caldicellulosiruptor saccharolyticus | |
| 1.21 | - |
2-nitrophenyl beta-D-galactopyranoside | at pH 6.0 and 80°C | Caldicellulosiruptor saccharolyticus | |
| 30 | - |
lactose | at pH 6.0 and 80°C | Caldicellulosiruptor saccharolyticus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 79711 | - |
3 * 79711, calculated from amino acid sequence | Caldicellulosiruptor saccharolyticus |
| 80000 | - |
3 * 80000, SDS-PAGE | Caldicellulosiruptor saccharolyticus |
| 240000 | - |
gel filtration | Caldicellulosiruptor saccharolyticus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Caldicellulosiruptor saccharolyticus | - |
strain DSM 8903 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| His-Trap column chromatography | Caldicellulosiruptor saccharolyticus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 21.7 | - |
crude extract, at pH 6.0 and 80°C | Caldicellulosiruptor saccharolyticus |
| 212 | - |
after 9.8fold purification, at pH 6.0 and 80°C | Caldicellulosiruptor saccharolyticus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-nitrophenyl beta-D-galactopyranoside + H2O | - |
Caldicellulosiruptor saccharolyticus | 2-nitrophenol + beta-D-galactose | - |
? | |
| 4-nitrophenyl beta-D-galactopyranoside + H2O | - |
Caldicellulosiruptor saccharolyticus | 4-nitrophenol + beta-D-galactose | - |
? | |
| lactose + H2O | - |
Caldicellulosiruptor saccharolyticus | D-galactose + D-glucose | - |
? | |
| additional information | the enzyme does no show activity for 4-nitrophenyl beta-D-glucopyranoside, 2-nitrophenyl beta-D-glucopyranoside, 4-nitrophenyl beta D-mannopyranoside, 4-nitrophenyl beta-D-xylopyranoside, 4-nitrophenyl alpha-L-arabinopyranoside, or cellobiose | Caldicellulosiruptor saccharolyticus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homotrimer | 3 * 79711, calculated from amino acid sequence | Caldicellulosiruptor saccharolyticus |
| homotrimer | 3 * 80000, SDS-PAGE | Caldicellulosiruptor saccharolyticus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
- |
Caldicellulosiruptor saccharolyticus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | 85 | at 65°C and 85°C the activity is approximately 60% of the maximum | Caldicellulosiruptor saccharolyticus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | 75 | the half-lives of the enzyme at 65°C, 70°C, 75°C, and 80°C are 128, 48, 17, and 2 h respectively | Caldicellulosiruptor saccharolyticus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 32 | - |
4-nitrophenyl beta-D-galactopyranoside | at pH 6.0 and 80°C | Caldicellulosiruptor saccharolyticus | |
| 42 | - |
lactose | at pH 6.0 and 80°C | Caldicellulosiruptor saccharolyticus | |
| 149 | - |
2-nitrophenyl beta-D-galactopyranoside | at pH 6.0 and 80°C | Caldicellulosiruptor saccharolyticus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Caldicellulosiruptor saccharolyticus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 7 | at pH 5.0 and 7.0 the activity is approximately 60% of the maximum | Caldicellulosiruptor saccharolyticus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 12 | - |
D-galactose | at pH 6.0 and 80°C | Caldicellulosiruptor saccharolyticus | |
| 1170 | - |
D-glucose | at pH 6.0 and 80°C | Caldicellulosiruptor saccharolyticus | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.4 | - |
lactose | at pH 6.0 and 80°C | Caldicellulosiruptor saccharolyticus | |
| 123 | - |
2-nitrophenyl beta-D-galactopyranoside | at pH 6.0 and 80°C | Caldicellulosiruptor saccharolyticus | |
| 296 | - |
4-nitrophenyl beta-D-galactopyranoside | at pH 6.0 and 80°C | Caldicellulosiruptor saccharolyticus | |
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