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Literature summary for 3.2.1.23 extracted from

  • Moracci, M.; La Volpe, A.; Pulitzer, J.F.; Rossi, M.; Ciaramella, M.
    Expression of the thermostable beta-galactosidase gene from the archaebacterium Sulfolobus solfataricus in Saccharomyces cerevisiae and characterization of a new inducible promoter for heterologous expression. (1992), J. Bacteriol., 174, 873-882.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene lacS, expression in Saccharomyces cerevisiae strain ScKY117 using a galactose-inducible promoter, the strong, heat-inducible upstream activating RcC9 sequence of the yeast genes GAL1 and GAL10, originally isolated from Caenorhabditis elegans, transcription of the lacS gene under the control of this sequence is rapidly and efficiently induced by heat shock, the recombinant enzyme is identical in molecular mass, thermostability, and thermophilicity to the native enzyme, heat induction by growth at 39°C for 45 min, overview Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus
-
gene lacS
-

Purification (Commentary)

Purification (Comment) Organism
recombinant soluble enzyme partially from Saccharomyces cerevisiae by heat treatment at 75°C for 30 min, and ultrafiltration Saccharolobus solfataricus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.72
-
partially purified enzyme, 37°C Saccharolobus solfataricus
16
-
partially purified enzyme, 75°C Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-nitrophenyl beta-D-galactopyranoside + H2O
-
Saccharolobus solfataricus 2-nitrophenol + beta-D-galactose
-
?
5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside + H2O
-
Saccharolobus solfataricus 5-bromo-4-chloro-indol + beta-D-galactose
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
90
-
above Saccharolobus solfataricus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
75
-
partially purified enzyme, 2 h, completely stable Saccharolobus solfataricus