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Literature summary for 3.2.1.23 extracted from
- Catalytic properties of a mutant beta-galactosidase from Xanthomonas manihotis engineered to synthesize galactosyl-thio-beta-1,3 and -beta-1,4-glycosides (2006), FEBS Lett., 580, 4377-4381.
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | enzyme mutant E184A is a valuable catalyst for the synthesis of metabolically stable analogues of the important glycosidic linkages to the 3 and 4 positions of glucosides and galactosides | Xanthomonas phaseoli pv. manihotis |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of mutant enzymes in Escherichia coli | Xanthomonas phaseoli pv. manihotis |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| E184A | site-directed mutagenesis, the mutant functions as an efficient thioglycoligase, which synthesises thiogalactosides with linkages to the 3 and 4 positions of glucosides and galactosides in high yields, the mutant shows reduced galactohydrolase activity compared to the wild-type enzyme | Xanthomonas phaseoli pv. manihotis |
| E184Q | site-directed mutagenesis, the mutant does not function as a thioglycoligase, the mutant shows reduced galactohydrolase activity compared to the wild-type enzyme | Xanthomonas phaseoli pv. manihotis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics of the thioglycoligase activity of mutant E184A, overview | Xanthomonas phaseoli pv. manihotis | |
| 0.00008 | - |
3,4-dinitrophenyl beta-D-galactopyranoside | pH 7.0, 30°C, mutant E184A, glycohydrolase activity | Xanthomonas phaseoli pv. manihotis |  |
| 0.002 | - |
4-nitrophenyl-beta-D-galactopyranoside | pH 7.0, 30°C, mutant E184A, glycohydrolase activity | Xanthomonas phaseoli pv. manihotis | |
| 0.05 | - |
4-nitrophenyl-beta-D-galactopyranoside | pH 7.0, 30°C, wild-type enzyme, glycohydrolase activity | Xanthomonas phaseoli pv. manihotis | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Xanthomonas phaseoli pv. manihotis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant mutant enzymes from Escherichia coli by ammonium sulfate fractionation and hydrophobic interaction chromatography | Xanthomonas phaseoli pv. manihotis |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| beta-D-galactopyranosyl-(1-4)-beta-D-galactopyranosyl-(1-6)-beta-D-galactopyranosyl-(1-3)-beta-D-galactopyranose + 3 H2O = 4 beta-D-galactopyranose | Glu184 acts as acid/base catalyst | Xanthomonas phaseoli pv. manihotis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3,4-dinitrophenyl beta-D-galactopyranoside + H2O | - |
Xanthomonas phaseoli pv. manihotis | 3,4-dinitrophenol + beta-D-galactose | - |
? | |
| 4-nitrophenyl beta-D-galactopyranoside + H2O | - |
Xanthomonas phaseoli pv. manihotis | 4-nitrophenol + beta-D-galactose | - |
? | |
| additional information | the mutant E184A enzyme also acts as thioglycoligase, reaction mechanism, overview | Xanthomonas phaseoli pv. manihotis | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| BgaX | - |
Xanthomonas phaseoli pv. manihotis |
| More | the enzyme belongs to the glycosyl hydrolase family 35 | Xanthomonas phaseoli pv. manihotis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
thioglycoligase assay at | Xanthomonas phaseoli pv. manihotis |
| 30 | - |
glycohydrolase assay at | Xanthomonas phaseoli pv. manihotis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.1 | - |
4-nitrophenyl-beta-D-galactopyranoside | pH 7.0, 30°C, mutant E184A, glycohydrolase activity | Xanthomonas phaseoli pv. manihotis | |
| 2.6 | - |
3,4-dinitrophenyl beta-D-galactopyranoside | pH 7.0, 30°C, mutant E184A, glycohydrolase activity | Xanthomonas phaseoli pv. manihotis | |
| 2140 | - |
4-nitrophenyl-beta-D-galactopyranoside | pH 7.0, 30°C, wild-type enzyme, glycohydrolase activity | Xanthomonas phaseoli pv. manihotis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Xanthomonas phaseoli pv. manihotis |
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