Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | slight activation | Saccharolobus solfataricus | |
cysteine | slight activation | Saccharolobus solfataricus | |
dithiothreitol | slight activation | Saccharolobus solfataricus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-aminophenyl beta-D-thiogalactopyranoside | competitive inhibition | Saccharolobus solfataricus | |
beta-D-glucose | product inhibition | Saccharolobus solfataricus | |
D-galactal | - |
Saccharolobus solfataricus | |
additional information | no inhibition by 4-chloromercuribenzoate or N-ethylmaleimide, and by D-galactose and isopropyl beta-D-thiogalactopyranoside | Saccharolobus solfataricus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics and thermodynamics | Saccharolobus solfataricus | |
0.225 | - |
2-nitrophenyl-beta-D-galactopyranoside | pH 6.5, 75°C | Saccharolobus solfataricus | |
2 | 3 | methyl-beta-D-galactopyranoside | pH 6.5, 30°C | Saccharolobus solfataricus | |
2.6 | - |
phenyl-beta-D-galactopyranoside | pH 6.5, 30°C | Saccharolobus solfataricus | |
13 | - |
lactose | pH 6.5, 30°C | Saccharolobus solfataricus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
4 * 60000, SDS-PAGE | Saccharolobus solfataricus |
220000 | - |
glycerol gradient centrifugation | Saccharolobus solfataricus |
240000 | - |
gel filtration | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | - |
- |
- |
Saccharolobus solfataricus MT-4 / DSM 5833 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
soluble, native enzyme 966fold by anion exchange chromatography, precipitation at pH 4.5, dialysis, 4-aminophenyl-beta-D-thiogalactopyranoside affinity chromatography, and another step of anion exchange chromatography to homogeneity | Saccharolobus solfataricus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
116.4 | - |
purified enzyme | Saccharolobus solfataricus |
Storage Stability | Organism |
---|---|
-20°C, purified enzyme, 50 mM sodium phosphate, pH 6.5, and 2.8 mM 4-nitrophenyl-beta-D-galactopyranoside, stable for long periods of storage | Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-nitrophenyl beta-D-galactopyranoside + H2O | - |
Saccharolobus solfataricus | 2-nitrophenol + beta-D-galactose | - |
? | |
2-nitrophenyl beta-D-galactopyranoside + H2O | - |
Saccharolobus solfataricus MT-4 / DSM 5833 | 2-nitrophenol + beta-D-galactose | - |
? | |
lactose + H2O | - |
Saccharolobus solfataricus | beta-D-glucose + beta-D-galactose | - |
? | |
lactose + H2O | - |
Saccharolobus solfataricus MT-4 / DSM 5833 | beta-D-glucose + beta-D-galactose | - |
? | |
methyl-beta-D-galactopyranoside + H2O | - |
Saccharolobus solfataricus | methanol + beta-D-galactose | - |
? | |
methyl-beta-D-galactopyranoside + H2O | - |
Saccharolobus solfataricus MT-4 / DSM 5833 | methanol + beta-D-galactose | - |
? | |
additional information | the enzyme displays a quite broad specificity, showing wide tolerance regarding the structure of the aglycon moiety, overview | Saccharolobus solfataricus | ? | - |
? | |
additional information | the enzyme displays a quite broad specificity, showing wide tolerance regarding the structure of the aglycon moiety, overview | Saccharolobus solfataricus MT-4 / DSM 5833 | ? | - |
? | |
phenyl-beta-D-galactopyranoside + H2O | - |
Saccharolobus solfataricus | phenol + beta-D-galactose | - |
? | |
phenyl-beta-D-galactopyranoside + H2O | - |
Saccharolobus solfataricus MT-4 / DSM 5833 | phenol + beta-D-galactose | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | enzyme amino acid composition, overview | Saccharolobus solfataricus |
tetramer | 4 * 60000, SDS-PAGE | Saccharolobus solfataricus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
95 | - |
- |
Saccharolobus solfataricus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 95 | temperature profile, 3% of maximal activity at 30°C | Saccharolobus solfataricus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
purified enzyme, pH 6.5, half-life is 24 h | Saccharolobus solfataricus |
80 | - |
purified enzyme, pH 6.5, half-life is 10 h | Saccharolobus solfataricus |
85 | - |
purified enzyme, pH 6.5, half-life is 3 h | Saccharolobus solfataricus |
95 | - |
purified enzyme, pH 6.5, complete loss of activity after 120 min | Saccharolobus solfataricus |
100 | - |
purified enzyme, pH 6.5, complete loss of activity after 15 min | Saccharolobus solfataricus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
114 | - |
lactose | pH 6.5, 30°C | Saccharolobus solfataricus | |
166 | - |
phenyl-beta-D-galactopyranoside | pH 6.5, 30°C | Saccharolobus solfataricus | |
242 | - |
methyl-beta-D-galactopyranoside | pH 6.5, 30°C | Saccharolobus solfataricus | |
5700 | - |
2-nitrophenyl-beta-D-galactopyranoside | pH 6.5, 75°C | Saccharolobus solfataricus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
assay at | Saccharolobus solfataricus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3.5 | 10 | pH profile | Saccharolobus solfataricus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
4 | - |
4-aminophenyl beta-D-thiogalactopyranoside | pH 6.5, 75°C | Saccharolobus solfataricus | |
12 | - |
D-galactal | pH 6.5, 75°C | Saccharolobus solfataricus | |
96 | - |
beta-D-glucose | pH 6.5, 75°C | Saccharolobus solfataricus |