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Literature summary for 3.2.1.23 extracted from

  • Pisani, F.M.; Rella, R.; Raia, C.A.; Rozzo, C.; Nucci, R.; Gambacorta, A.; De Rosa, M.; Rossi, M.
    Thermostable beta-galactosidase from the archaebacterium Sulfolobus solfataricus. Purification and properties (1990), Eur. J. Biochem., 187, 321-328.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol slight activation Saccharolobus solfataricus
cysteine slight activation Saccharolobus solfataricus
dithiothreitol slight activation Saccharolobus solfataricus

Inhibitors

Inhibitors Comment Organism Structure
4-aminophenyl beta-D-thiogalactopyranoside competitive inhibition Saccharolobus solfataricus
beta-D-glucose product inhibition Saccharolobus solfataricus
D-galactal
-
Saccharolobus solfataricus
additional information no inhibition by 4-chloromercuribenzoate or N-ethylmaleimide, and by D-galactose and isopropyl beta-D-thiogalactopyranoside Saccharolobus solfataricus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics and thermodynamics Saccharolobus solfataricus
0.225
-
2-nitrophenyl-beta-D-galactopyranoside pH 6.5, 75°C Saccharolobus solfataricus
2 3 methyl-beta-D-galactopyranoside pH 6.5, 30°C Saccharolobus solfataricus
2.6
-
phenyl-beta-D-galactopyranoside pH 6.5, 30°C Saccharolobus solfataricus
13
-
lactose pH 6.5, 30°C Saccharolobus solfataricus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
4 * 60000, SDS-PAGE Saccharolobus solfataricus
220000
-
glycerol gradient centrifugation Saccharolobus solfataricus
240000
-
gel filtration Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus
-
-
-
Saccharolobus solfataricus MT-4 / DSM 5833
-
-
-

Purification (Commentary)

Purification (Comment) Organism
soluble, native enzyme 966fold by anion exchange chromatography, precipitation at pH 4.5, dialysis, 4-aminophenyl-beta-D-thiogalactopyranoside affinity chromatography, and another step of anion exchange chromatography to homogeneity Saccharolobus solfataricus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
116.4
-
purified enzyme Saccharolobus solfataricus

Storage Stability

Storage Stability Organism
-20°C, purified enzyme, 50 mM sodium phosphate, pH 6.5, and 2.8 mM 4-nitrophenyl-beta-D-galactopyranoside, stable for long periods of storage Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-nitrophenyl beta-D-galactopyranoside + H2O
-
Saccharolobus solfataricus 2-nitrophenol + beta-D-galactose
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
-
Saccharolobus solfataricus MT-4 / DSM 5833 2-nitrophenol + beta-D-galactose
-
?
lactose + H2O
-
Saccharolobus solfataricus beta-D-glucose + beta-D-galactose
-
?
lactose + H2O
-
Saccharolobus solfataricus MT-4 / DSM 5833 beta-D-glucose + beta-D-galactose
-
?
methyl-beta-D-galactopyranoside + H2O
-
Saccharolobus solfataricus methanol + beta-D-galactose
-
?
methyl-beta-D-galactopyranoside + H2O
-
Saccharolobus solfataricus MT-4 / DSM 5833 methanol + beta-D-galactose
-
?
additional information the enzyme displays a quite broad specificity, showing wide tolerance regarding the structure of the aglycon moiety, overview Saccharolobus solfataricus ?
-
?
additional information the enzyme displays a quite broad specificity, showing wide tolerance regarding the structure of the aglycon moiety, overview Saccharolobus solfataricus MT-4 / DSM 5833 ?
-
?
phenyl-beta-D-galactopyranoside + H2O
-
Saccharolobus solfataricus phenol + beta-D-galactose
-
?
phenyl-beta-D-galactopyranoside + H2O
-
Saccharolobus solfataricus MT-4 / DSM 5833 phenol + beta-D-galactose
-
?

Subunits

Subunits Comment Organism
More enzyme amino acid composition, overview Saccharolobus solfataricus
tetramer 4 * 60000, SDS-PAGE Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
95
-
-
Saccharolobus solfataricus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
35 95 temperature profile, 3% of maximal activity at 30°C Saccharolobus solfataricus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
75
-
purified enzyme, pH 6.5, half-life is 24 h Saccharolobus solfataricus
80
-
purified enzyme, pH 6.5, half-life is 10 h Saccharolobus solfataricus
85
-
purified enzyme, pH 6.5, half-life is 3 h Saccharolobus solfataricus
95
-
purified enzyme, pH 6.5, complete loss of activity after 120 min Saccharolobus solfataricus
100
-
purified enzyme, pH 6.5, complete loss of activity after 15 min Saccharolobus solfataricus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
114
-
lactose pH 6.5, 30°C Saccharolobus solfataricus
166
-
phenyl-beta-D-galactopyranoside pH 6.5, 30°C Saccharolobus solfataricus
242
-
methyl-beta-D-galactopyranoside pH 6.5, 30°C Saccharolobus solfataricus
5700
-
2-nitrophenyl-beta-D-galactopyranoside pH 6.5, 75°C Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
assay at Saccharolobus solfataricus

pH Range

pH Minimum pH Maximum Comment Organism
3.5 10 pH profile Saccharolobus solfataricus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4
-
4-aminophenyl beta-D-thiogalactopyranoside pH 6.5, 75°C Saccharolobus solfataricus
12
-
D-galactal pH 6.5, 75°C Saccharolobus solfataricus
96
-
beta-D-glucose pH 6.5, 75°C Saccharolobus solfataricus