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Literature summary for 3.2.1.23 extracted from
- The effect of physico-chemical parameters and chemical compounds on the activity of beta-D-galactosidase (B-GAL), a marker enzyme for indicator microorganisms in water (2007), Chemosphere, 68, 622-627.
Application
| Application | Comment | Organism |
|---|---|---|
| environmental protection | the presence of coliforms in polluted water is determined enzymatically in situ by directly monitoring the activity of B-GAL through the hydrolysis of the yellow chromogenic subtrate, chlorophenol red beta-D-galactopyranoside, which produces a red chlorophenol red product, assay evaluation, overview | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | - |
Escherichia coli |  |
| Ca2+ | activates at 50-100 mg/l, inhibits at concentrations above 200 mg/l | Escherichia coli | |
| Carbonate | - |
Escherichia coli | |
| chloride | - |
Escherichia coli | |
| Citric acid | slight inhibition at 200 mg/l | Escherichia coli | |
| Cu2+ | - |
Escherichia coli | |
| EDTA | - |
Escherichia coli | |
| Fe2+ | activates at 50-100 mg/l, inhibits at concentrations above 200 mg/l | Escherichia coli | |
| ferulic acid | - |
Escherichia coli | |
| sodium hypochlorite | - |
Escherichia coli | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | activates at 50-100 mg/l, inhibits at concentrations above 200 mg/l | Escherichia coli | |
| Fe2+ | activates at 50-100 mg/l, inhibits at concentrations above 200 mg/l | Escherichia coli | |
| Mg2+ | activates at 50-200 mg/l | Escherichia coli | |
| nitrate | activates | Escherichia coli | |
| sulfate | activates | Escherichia coli | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| lactose + H2O | Escherichia coli | - |
D-glucose + D-galactose | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| chlorophenol red beta-D-galactopyranoside + H2O | - |
Escherichia coli | chlorophenol red + beta-D-galactose | - |
? | |
| lactose + H2O | - |
Escherichia coli | D-glucose + D-galactose | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| B-GAL | - |
Escherichia coli |
| beta-D-galactosidase | - |
Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
two optima, 35°C and 55°C | Escherichia coli |
| 55 | - |
two optima, 35°C and 55°C | Escherichia coli |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 65 | - |
Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | - |
- |
Escherichia coli |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 11 | 60% of maximal activity at pH 7.5 and pH 9.0 | Escherichia coli |
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Release 2023.1 (January 2023)
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