Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-aminophenyl beta-D-thiogalactopyranoside | - |
Priestia megaterium |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
120000 | - |
2 * 120000, SDS-PAGE | Priestia megaterium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Priestia megaterium | - |
- |
- |
Priestia megaterium KM / ATCC 13632 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme to homogeneity by 4-aminophenyl beta-D-thiogalactopyranoside affinity chromatography | Priestia megaterium |
Renatured (Comment) | Organism |
---|---|
denatured protein from 8 M urea is renatured by dialysis and addition of free and active monomer | Priestia megaterium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-nitrophenyl beta-D-galactopyranoside + H2O | - |
Priestia megaterium | 2-nitrophenol + beta-D-galactose | - |
? | |
2-nitrophenyl beta-D-galactopyranoside + H2O | - |
Priestia megaterium KM / ATCC 13632 | 2-nitrophenol + beta-D-galactose | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 120000, SDS-PAGE | Priestia megaterium |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
31 | - |
assay at | Priestia megaterium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Priestia megaterium |