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Literature summary for 3.2.1.23 extracted from

  • Adalberto, P.R.; Massabni, A.C.; Goulart, A.J.; Contiero, J.; Carmona, E.C.; Cardello, L.; Monti, R.
    Production of beta-galactosidase by Trichoderma reesei FTKO-39 in wheat bran: partial purification of two isozymes (2006), Appl. Biochem. Biotechnol., 133, 163-170.
    View publication on PubMed
Search for more literature for 3.2.1.23

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.65
-
 2-nitrophenyl-beta-D-galactopyranoside pH 7.5, 30°C, isozyme BGT I Trichoderma reesei
2.66
-
 lactose pH 7.5, 35°C, isozyme BGT I Trichoderma reesei
19.01
-
 lactose pH 7.5, 35°C, isozyme BGT II Trichoderma reesei
21.68
-
 2-nitrophenyl-beta-D-galactopyranoside pH 7.5, 30°C, isozyme BGT II Trichoderma reesei

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Trichoderma reesei

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
lactose + H2O Trichoderma reesei
-
 D-glucose + D-galactose
-
 ?
lactose + H2O Trichoderma reesei FTKO-39
-
 D-glucose + D-galactose
-
 ?

Organism

Organism UniProt Comment Textmining
Trichoderma reesei
-
 two isozymes BGT I and BGT II
-
Trichoderma reesei FTKO-39
-
 two isozymes BGT I and BGT II
-

Purification (Commentary)

Purification (Comment) Organism
native isozymes BGT I and BGT II by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration, isozyme BGT I id purified 13fold, and isozyme BGT II 8.4fold Trichoderma reesei

Source Tissue

Source Tissue Comment Organism Textmining
additional information culture is grown on wheat bran supplemented with MgCl2 and lactose as the carbon source Trichoderma reesei
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
20.33
-
 purified isozyme BGT II Trichoderma reesei
31.25
-
 purified isozyme BGT I Trichoderma reesei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-nitrophenyl beta-D-galactopyranoside + H2O
-
 Trichoderma reesei 2-nitrophenol + beta-D-galactose
-
 ?
2-nitrophenyl beta-D-galactopyranoside + H2O
-
 Trichoderma reesei FTKO-39 2-nitrophenol + beta-D-galactose
-
 ?
lactose + H2O
-
 Trichoderma reesei D-glucose + D-galactose
-
 ?
lactose + H2O
-
 Trichoderma reesei FTKO-39 D-glucose + D-galactose
-
 ?

Synonyms

Synonyms Comment Organism
BGT I
-
 Trichoderma reesei
BGT II
-
 Trichoderma reesei

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
 isozymes BGT I and BGT II Trichoderma reesei

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 90
-
 Trichoderma reesei

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
65
-
 the half-lives are 128 min and 118 min for purified isozymes BGT I and BGT II, respectively Trichoderma reesei

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
 isozyme BGT I Trichoderma reesei
6.5
-
 isozyme BGT II Trichoderma reesei

pH Range

pH Minimum pH Maximum Comment Organism
3 8.5
-
 Trichoderma reesei

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 10 isozyme BGT I shows 90% remaining activity within this range after 30 min, while isozyme BGT II shows only 50% activity after 30 min Trichoderma reesei