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Literature summary for 3.2.1.23 extracted from

  • Cieslinski, H.; Kur, J.; Bialkowska, A.; Baran, I.; Makowski, K.; Turkiewicz, M.
    Cloning, expression, and purification of a recombinant cold-adapted beta-galactosidase from antarctic bacterium Pseudoalteromonas sp. 22b (2005), Protein Expr. Purif., 39, 27-34.
    View publication on PubMed
Search for more literature for 3.2.1.23

Application

Application Comment Organism
nutrition recombinant Pseudoalteromonas sp. 22b beta-galactosidase could be applied at refrigeration temperatures for production of lactose-reduced dairy products Pseudoalteromonas sp.

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pseudoalteromonas sp.

Organism

Organism UniProt Comment Textmining
Pseudoalteromonas sp. Q6RGS1
-
-
Pseudoalteromonas sp. 22b Q6RGS1
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudoalteromonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
lactose + H2O 90% of milk lactose is hydrolyzed during 6 h at 30°C and during 28 h at 15°C Pseudoalteromonas sp. D-glucose + D-galactose
-
 ?
lactose + H2O 90% of milk lactose is hydrolyzed during 6 h at 30°C and during 28 h at 15°C Pseudoalteromonas sp. 22b D-glucose + D-galactose
-
 ?
o-nitrophenyl-beta-D-galactoside + H2O
-
 Pseudoalteromonas sp. o-nitrophenol + beta-D-galactose
-
 ?
o-nitrophenyl-beta-D-galactoside + H2O
-
 Pseudoalteromonas sp. 22b o-nitrophenol + beta-D-galactose
-
 ?
p-nitrophenyl-beta-D-galactoside + H2O 58% higher activity than with o-nitrophenyl-beta-D-galactoside Pseudoalteromonas sp. p-nitrophenol + beta-D-galactose
-
 ?
p-nitrophenyl-beta-D-galactoside + H2O 58% higher activity than with o-nitrophenyl-beta-D-galactoside Pseudoalteromonas sp. 22b p-nitrophenol + beta-D-galactose
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Pseudoalteromonas sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 40 10°C: 20% of maximal activity, 40°C: optimum Pseudoalteromonas sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 10 min, complete loss of activity Pseudoalteromonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 8
-
 Pseudoalteromonas sp.