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Literature summary for 3.2.1.23 extracted from
- Quantitative assay of senescence-associated beta-galactosidase activity in mammalian cell extracts (2005), Anal. Biochem., 343, 329-334.
Application
| Application | Comment | Organism |
|---|---|---|
| molecular biology | senescence-associated beta-galactosidase activity is a widely used biomarker for assessing replicative sensescence in mammalian cells. Quantitative assay of senescence-associated beta-galactosidase activity in mammalian cell extracts. The assay is capable of detecting relatively subtle changes in activity and confirms that confluency and contact inhibition of growth can cause a small increase in the expression of this biomarker. The assay for measuring senescence-associated changes in beta-galactosidase is suitable for mechanistic studies of senescence regulation in which graduated changes in biomarker expression may be anticipated | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| fibroblast | confluency and contact inhibition of growth can cause a small increase in the expression. Senescent phenyotype is correlated with an increase in total beta-galactosidase | Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside + H2O | - |
Homo sapiens | ? | - |
? |  |
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Release 2023.1 (January 2023)
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