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Literature summary for 3.2.1.23 extracted from

  • Hoyoux, A.; Jennes, I.; Dubois, P.; Genicot, S.; Dubail, F.; Francois, J.M.; Baise, E.; Feller, G.; Gerday, C.
    Cold-adapted beta-galactosidase from the antarctic psychrophile Pseudoalteromonas haloplanktis (2001), Appl. Environ. Microbiol., 67, 1529-1535.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
nutrition cold-adapted beta-galactosidase could be used to hydrolyze lactose in dairy products processed in refrigerated plants Pseudoalteromonas haloplanktis

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pseudoalteromonas haloplanktis

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 1 mM, strong inhibition Pseudoalteromonas haloplanktis
EDTA 5 mM, 90% loss of activity. Excess of Mg2+, Mn2+, Li2+ and Ca2+ restores activity Pseudoalteromonas haloplanktis
Ni2+ 1 mM, strong inhibition Pseudoalteromonas haloplanktis
Zn2+ 1 mM, strong inhibition Pseudoalteromonas haloplanktis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4
-
lactose 25°C Pseudoalteromonas haloplanktis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
118000
-
x * 118000, SDS-PAGE Pseudoalteromonas haloplanktis

Organism

Organism UniProt Comment Textmining
Pseudoalteromonas haloplanktis P81650 TAE 79
-
Pseudoalteromonas haloplanktis TAE 79 P81650 TAE 79
-

Purification (Commentary)

Purification (Comment) Organism
-
Pseudoalteromonas haloplanktis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
138.2
-
-
Pseudoalteromonas haloplanktis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
lactose + H2O
-
Pseudoalteromonas haloplanktis D-glucose + D-galactose
-
?
lactose + H2O
-
Pseudoalteromonas haloplanktis TAE 79 D-glucose + D-galactose
-
?
o-nitrophenyl beta-D-galactopyranoside + H2O
-
Pseudoalteromonas haloplanktis o-nitrophenol + beta-D-galactose
-
?
o-nitrophenyl beta-D-galactopyranoside + H2O
-
Pseudoalteromonas haloplanktis TAE 79 o-nitrophenol + beta-D-galactose
-
?

Subunits

Subunits Comment Organism
? x * 118000, SDS-PAGE Pseudoalteromonas haloplanktis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
48
-
hydrolysis of o-nitrophenyl beta-D-galactopyranoside Pseudoalteromonas haloplanktis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
28 48 28°C: about 45% of maximal activity, 48°C: optimum, 50°C: about 5% of maximal activity, hydrolysis of o-nitrophenyl beta-D-galactopyranoside Pseudoalteromonas haloplanktis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
48
-
melting point of wild-type enzyme Pseudoalteromonas haloplanktis
50
-
melting point of recombinant enzyme Pseudoalteromonas haloplanktis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
33
-
lactose 25°C Pseudoalteromonas haloplanktis
203
-
o-nitrophenyl beta-D-galactopyranoside 20°C Pseudoalteromonas haloplanktis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
hydrolysis of o-nitrophenyl beta-D-galactopyranoside Pseudoalteromonas haloplanktis

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.5 10 stable Pseudoalteromonas haloplanktis

pI Value

Organism Comment pI Value Maximum pI Value
Pseudoalteromonas haloplanktis isoelectric focusing
-
6.9