Application | Comment | Organism |
---|---|---|
nutrition | cold-adapted beta-galactosidase could be used to hydrolyze lactose in dairy products processed in refrigerated plants | Pseudoalteromonas haloplanktis |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pseudoalteromonas haloplanktis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 1 mM, strong inhibition | Pseudoalteromonas haloplanktis | |
EDTA | 5 mM, 90% loss of activity. Excess of Mg2+, Mn2+, Li2+ and Ca2+ restores activity | Pseudoalteromonas haloplanktis | |
Ni2+ | 1 mM, strong inhibition | Pseudoalteromonas haloplanktis | |
Zn2+ | 1 mM, strong inhibition | Pseudoalteromonas haloplanktis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.4 | - |
lactose | 25°C | Pseudoalteromonas haloplanktis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
118000 | - |
x * 118000, SDS-PAGE | Pseudoalteromonas haloplanktis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudoalteromonas haloplanktis | P81650 | TAE 79 | - |
Pseudoalteromonas haloplanktis TAE 79 | P81650 | TAE 79 | - |
Purification (Comment) | Organism |
---|---|
- |
Pseudoalteromonas haloplanktis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
138.2 | - |
- |
Pseudoalteromonas haloplanktis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
lactose + H2O | - |
Pseudoalteromonas haloplanktis | D-glucose + D-galactose | - |
? | |
lactose + H2O | - |
Pseudoalteromonas haloplanktis TAE 79 | D-glucose + D-galactose | - |
? | |
o-nitrophenyl beta-D-galactopyranoside + H2O | - |
Pseudoalteromonas haloplanktis | o-nitrophenol + beta-D-galactose | - |
? | |
o-nitrophenyl beta-D-galactopyranoside + H2O | - |
Pseudoalteromonas haloplanktis TAE 79 | o-nitrophenol + beta-D-galactose | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 118000, SDS-PAGE | Pseudoalteromonas haloplanktis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
48 | - |
hydrolysis of o-nitrophenyl beta-D-galactopyranoside | Pseudoalteromonas haloplanktis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
28 | 48 | 28°C: about 45% of maximal activity, 48°C: optimum, 50°C: about 5% of maximal activity, hydrolysis of o-nitrophenyl beta-D-galactopyranoside | Pseudoalteromonas haloplanktis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
48 | - |
melting point of wild-type enzyme | Pseudoalteromonas haloplanktis |
50 | - |
melting point of recombinant enzyme | Pseudoalteromonas haloplanktis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
33 | - |
lactose | 25°C | Pseudoalteromonas haloplanktis | |
203 | - |
o-nitrophenyl beta-D-galactopyranoside | 20°C | Pseudoalteromonas haloplanktis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
hydrolysis of o-nitrophenyl beta-D-galactopyranoside | Pseudoalteromonas haloplanktis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6.5 | 10 | stable | Pseudoalteromonas haloplanktis |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Pseudoalteromonas haloplanktis | isoelectric focusing | - |
6.9 |