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Literature summary for 3.2.1.23 extracted from

  • Choi, Y.J.; Kim, I.H.; Lee, B.H.; Lee, J.S.
    Purification and characterization of beta-galactosidase from alkalophilic and thermophilic Bacillus sp. TA-11 (1995), Biotechnol. Appl. Biochem., 22, 191-201.
No PubMed abstract available
Search for more literature for 3.2.1.23

Inhibitors

Inhibitors Comment Organism Structure
Al3+ 1 mM, 93% inhibition Bacillus sp. (in: Bacteria)
Cd2+ 1 mM, 94% inhibition Bacillus sp. (in: Bacteria)
cellobiose 10 mM, 30% inhibition Bacillus sp. (in: Bacteria)
Co2+ 1 mM, 54% inhibition Bacillus sp. (in: Bacteria)
D-galactose 10 mM, 40% loss of activity Bacillus sp. (in: Bacteria)
D-glucose 10 mM, 30% inhibition Bacillus sp. (in: Bacteria)
EDTA
-
 Bacillus sp. (in: Bacteria)
Fe2+ 1 mM, 20% inhibition Bacillus sp. (in: Bacteria)
Hg2+ 1 mM, 95% inhibition Bacillus sp. (in: Bacteria)
maltose 10 mM, 24% loss of activity Bacillus sp. (in: Bacteria)
starch 0.2% soluble starch Bacillus sp. (in: Bacteria)
xylose 10 mM, 30% inhibition Bacillus sp. (in: Bacteria)
Zn2+ 1 mM, 74% inhibition Bacillus sp. (in: Bacteria)

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
13.5
-
 o-nitrophenyl-beta-D-galactoside
-
 Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
200000
-
 gel filtration Bacillus sp. (in: Bacteria)

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria)
-
-
-
Bacillus sp. (in: Bacteria) TA-11
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bacillus sp. (in: Bacteria)

Source Tissue

Source Tissue Comment Organism Textmining
additional information cells from late-exponential phase Bacillus sp. (in: Bacteria)
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
p-nitrophenyl-beta-D-galactoside + H2O
-
 Bacillus sp. (in: Bacteria) p-nitrophenol + beta-D-galactose
-
 ?
p-nitrophenyl-beta-D-galactoside + H2O
-
 Bacillus sp. (in: Bacteria) TA-11 p-nitrophenol + beta-D-galactose
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 1 h, stable Bacillus sp. (in: Bacteria)
50
-
 1 h, about 20% loss of activity Bacillus sp. (in: Bacteria)
60
-
 1 h, about 60% loss of activity Bacillus sp. (in: Bacteria)
70
-
 1 h, complete inactivation Bacillus sp. (in: Bacteria)

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Bacillus sp. (in: Bacteria)

pH Range

pH Minimum pH Maximum Comment Organism
4 8 pH 4.0: about 30% of maximal activity, pH 8.0: about 45% of maximal activity Bacillus sp. (in: Bacteria)