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Literature summary for 3.2.1.23 extracted from

  • Etcheberrigaray, J.L.; Vattuone, M.A.; Sampietro, A.R.
    beta-Galactosidase from sugar cane (1986), Phytochemistry, 20, 49-51.
No PubMed abstract available
Search for more literature for 3.2.1.23

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol 75 mM, 13% activation Saccharum officinarum
Cys 16-24 mM, 10% activation Saccharum officinarum
ethanol 0.84 M, 32% activation Saccharum officinarum
sucrose 350 mM, 5% activation Saccharum officinarum

Inhibitors

Inhibitors Comment Organism Structure
Cys 8 mM, 7.5% inhibition Saccharum officinarum
Hg2+ 0.005 mM, 64.5% inhibition Saccharum officinarum
sucrose 30 mM, 10% inhibition Saccharum officinarum

Localization

Localization Comment Organism GeneOntology No. Textmining
cell wall most of the activity is associated with Saccharum officinarum 5618
-
intracellular 12-16% of the activity Saccharum officinarum 5622
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 30 mM, 6% activation Saccharum officinarum
Mg2+ 30 mM, 20% activation Saccharum officinarum
Mn2+ 30 mM, 25% activation Saccharum officinarum
Zn2+ 30 mM, 10% activation Saccharum officinarum

Organism

Organism UniProt Comment Textmining
Saccharum officinarum
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf blades and sheaths Saccharum officinarum
-
root elongation zone and hair zone Saccharum officinarum
-
stem
-
 Saccharum officinarum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
lactose + H2O
-
 Saccharum officinarum D-glucose + D-galactose
-
 ?
o-nitrophenyl-beta-D-galactoside + H2O
-
 Saccharum officinarum o-nitrophenol + beta-D-galactose
-
 ?
p-nitrophenyl-beta-D-galactoside + H2O
-
 Saccharum officinarum p-nitrophenol + beta-D-galactose
-
 ?
phenyl-beta-D-galactoside + H2O
-
 Saccharum officinarum phenol + beta-D-galactose
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
47
-
 pH 4, 60 min, 42% loss of activity Saccharum officinarum
50
-
 50 min, complete inactivation Saccharum officinarum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.3
-
-
 Saccharum officinarum

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.5 6.7 37°C, maximal stability Saccharum officinarum