Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.23 extracted from

  • Buonocore, V.; Sgambati, O.; de Rosa, M.; Esposito, E.; Gambacorta, A.
    A constitutive beta-galactosidase from the extreme thermoacidophile archaebacterium Caldariella acidophila: properties of the enzyme in the free state and in immobilized whole cells (1980), J. Appl. Biochem., 2, 390-397.
No PubMed abstract available
Search for more literature for 3.2.1.23

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.67
-
 4-nitrophenyl beta-D-galactopyranoside
-
 Caldariella acidophila
61.5
-
 lactose
-
 Caldariella acidophila

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Caldariella acidophila constitutive enzyme ?
-
 ?

Organism

Organism UniProt Comment Textmining
Caldariella acidophila
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Caldariella acidophila

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
11.6
-
-
 Caldariella acidophila

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-D-galactopyranoside + H2O
-
 Caldariella acidophila 4-nitrophenol + beta-D-galactopyranose
-
 ?
lactose + H2O
-
 Caldariella acidophila D-glucose + D-galactose
-
 ?
additional information constitutive enzyme Caldariella acidophila ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
-
 Caldariella acidophila

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 half-life: 550 h, half life of the enzyme in whole cells entrapped in a polyacrylamide gel in presence of lactose: 28 days Caldariella acidophila
85
-
 half-life: 550 h Caldariella acidophila

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
 hydrolysis of p-nitrophenyl-beta-D-galactoside Caldariella acidophila
5 6 hydrolysis of lactose Caldariella acidophila