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Literature summary for 3.2.1.23 extracted from

  • Levin, R.E.; Mahoney, R.R.
    Purification and characterization of beta-galactosidase from a strain of Bacillus coagulans (1981), Antonie van Leeuwenhoek, 47, 53-64.
    View publication on PubMed
Search for more literature for 3.2.1.23

Activating Compound

Activating Compound Comment Organism Structure
EDTA 0.001 M, 23% increase of activity Weizmannia coagulans

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 0.001 M, complete inhibition Weizmannia coagulans
D-galactose competitive Weizmannia coagulans
dextrose noncompetitive Weizmannia coagulans
Fe2+ 0.001 M, complete inhibition Weizmannia coagulans
Fe3+ 0.001 M, complete inhibition Weizmannia coagulans
Hg2+ 0.001 M, complete inhibition Weizmannia coagulans
maltose
-
 Weizmannia coagulans
raffinose
-
 Weizmannia coagulans
sorbose
-
 Weizmannia coagulans
xylose
-
 Weizmannia coagulans
Zn2+ 0.001 M, 47% loss of activity Weizmannia coagulans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2
-
 o-nitrophenyl-beta-D-galactoside at 55°C Weizmannia coagulans
4.2 5.6 o-nitrophenyl-beta-D-galactoside
-
 Weizmannia coagulans
5.6
-
 o-nitrophenyl-beta-D-galactoside at 30°C Weizmannia coagulans
50
-
 lactose at 55°C Weizmannia coagulans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
430000
-
 gel filtration Weizmannia coagulans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Weizmannia coagulans constitutive enzyme ?
-
 ?
additional information Weizmannia coagulans L4 constitutive enzyme ?
-
 ?

Organism

Organism UniProt Comment Textmining
Weizmannia coagulans
-
-
-
Weizmannia coagulans L4
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Weizmannia coagulans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Weizmannia coagulans

Storage Stability

Storage Stability Organism
4°C, 72 h, stable Weizmannia coagulans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
lactose + H2O
-
 Weizmannia coagulans D-glucose + D-galactose
-
 ?
lactose + H2O
-
 Weizmannia coagulans L4 D-glucose + D-galactose
-
 ?
additional information constitutive enzyme Weizmannia coagulans ?
-
 ?
additional information constitutive enzyme Weizmannia coagulans L4 ?
-
 ?
o-nitrophenyl-beta-D-galactoside + H2O
-
 Weizmannia coagulans o-nitrophenol + beta-D-galactose
-
 ?
o-nitrophenyl-beta-D-galactoside + H2O
-
 Weizmannia coagulans L4 o-nitrophenol + beta-D-galactose
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 hydrolysis of o-nitrophenyl-beta-D-galactoside Weizmannia coagulans
55
-
 hydrolysis of lactose Weizmannia coagulans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
 30 min, 70% loss of activity, 42% loss of activity in presence of 2 mg/ml bovine serum albumin Weizmannia coagulans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 hydrolysis of o-nitrophenyl-beta-D-galactoside Weizmannia coagulans
6.5
-
 hydrolysis of lactose Weizmannia coagulans