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Literature summary for 3.2.1.23 extracted from
- Purification and some properties of beta-galactosidase from Penicillium multicolor (1983), Agric. Biol. Chem., 47, 2533-2540.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | - |
Penicillium multicolor |  |
| Hg2+ | - |
Penicillium multicolor | |
| p-aminophenyl beta-D-thiogalactoside | - |
Penicillium multicolor | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.6 | - |
o-nitrophenyl-beta-D-galactoside | - |
Penicillium multicolor | |
| 8.9 | - |
lactose | - |
Penicillium multicolor | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 126000 | - |
gel filtration | Penicillium multicolor |
| 130000 | - |
1 * 130000, SDS-PAGE | Penicillium multicolor |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Penicillium multicolor | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | 15% of the carbohydrate is mannose | Penicillium multicolor |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Penicillium multicolor |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 145 | - |
- |
Penicillium multicolor |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| lactose + H2O | - |
Penicillium multicolor | D-glucose + D-galactose | - |
? | |
| o-nitrophenyl-beta-D-galactoside + H2O | - |
Penicillium multicolor | o-nitrophenol + beta-D-galactose | - |
? | |
| p-nitrophenyl-beta-D-galactoside + H2O | - |
Penicillium multicolor | p-nitrophenol + beta-D-galactose | - |
? | |
| phenyl-beta-D-galactoside + H2O | - |
Penicillium multicolor | phenol + beta-D-galactose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 130000, SDS-PAGE | Penicillium multicolor |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Penicillium multicolor |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 70 | 30°C: about 40% of maximal activity, 70°C: about 30% of maximal activity | Penicillium multicolor |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
pH 5.6, 15 min, retains more than 95% of its activity below | Penicillium multicolor |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
- |
Penicillium multicolor |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3.5 | 7.5 | 40°C, 3 h, stable | Penicillium multicolor |
| 5.6 | - |
room temperature, 10 days, retains more than 90% of maximal activity | Penicillium multicolor |
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