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Literature summary for 3.2.1.23 extracted from
- A novel thermostable extracellular beta-galactosidase of Paecilomyces varioti (1988), Appl. Microbiol. Biotechnol., 27, 383-388.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1-D-galactosylamine | mixed-type inhibition | Paecilomyces variotii |  |
| Ba2+ | 10 mM, 28% inhibition | Paecilomyces variotii | |
| Cu2+ | 10 mM, 28% inhibition | Paecilomyces variotii | |
| D-glucono-delta-lactone | mixed-type inhibition | Paecilomyces variotii | |
| Fe2+ | 10 mM, 20% inhibition | Paecilomyces variotii | |
| Hg2+ | 10 mM, complete inactivation | Paecilomyces variotii | |
| Ni2+ | 10 mM, 28% inhibition | Paecilomyces variotii | |
| p-hydroxymercuribenzoate | 1 mM, complete inactivation | Paecilomyces variotii | |
| PCMB | - |
Paecilomyces variotii | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 64 | - |
lactose | - |
Paecilomyces variotii | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Paecilomyces variotii | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 94000 | - |
gel filtration | Paecilomyces variotii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Paecilomyces variotii | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Paecilomyces variotii |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Paecilomyces variotii |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Paecilomyces variotii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cellobiose + H2O | 41.7% of the activity with lactose | Paecilomyces variotii | 2 D-glucose | - |
? | |
| gentiobiose + H2O | 14.3% of the activity with lactose | Paecilomyces variotii | 2 beta-D-glucose | - |
? | |
| lactose + H2O | - |
Paecilomyces variotii | D-glucose + D-galactose | - |
? | |
| o-nitrophenyl-beta-D-galactoside + H2O | weak activity | Paecilomyces variotii | o-nitrophenol + beta-D-galactose | - |
? | |
| p-nitrophenyl-beta-D-glucoside + H2O | 20.6% of the activity with lactose | Paecilomyces variotii | p-nitrophenol + beta-D-glucose | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Paecilomyces variotii |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
3 h, in presence of 4% w/v lactose, completely stable | Paecilomyces variotii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 3.5 | - |
- |
Paecilomyces variotii |
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Release 2023.1 (January 2023)
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