Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Triton X-100 | 0.01%, enhances activity | Raphanus sativus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | 1 mM, almost complete inhibition | Raphanus sativus | |
Cu2+ | 1 mM, almost complete inhibition | Raphanus sativus | |
D-arabinose | 10 mM, 15% inhibition | Raphanus sativus | |
D-fucose | 10 mM, 20% inhibition | Raphanus sativus | |
D-galactal | potent, competitive | Raphanus sativus | |
D-galactono-1,4-lactone | potent, competitive | Raphanus sativus | |
D-galactose | 10 mM D-galactose, 60% inhibition | Raphanus sativus | |
Fe3+ | 1 mM, almost complete inhibition | Raphanus sativus | |
Hg2+ | 1 mM, almost complete inhibition | Raphanus sativus | |
iodoacetate | 1 mM, 52% inhibition | Raphanus sativus | |
L-arabinose | 10 mM, 18% inhibition | Raphanus sativus | |
L-fucose | 10 mM, 30% inhibition | Raphanus sativus | |
Mn2+ | 1 mM, almost complete inhibition | Raphanus sativus | |
PCMB | - |
Raphanus sativus | |
SDS | 0.01%, complete inactivation | Raphanus sativus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.46 | - |
p-nitrophenyl-beta-D-galactoside | - |
Raphanus sativus | |
1.19 | - |
o-nitrophenyl-beta-D-galactoside | - |
Raphanus sativus | |
1.27 | - |
4-methylumbelliferyl-beta-D-galactoside | - |
Raphanus sativus | |
4.87 | - |
beta-1,3-galactotriose | - |
Raphanus sativus | |
7.79 | - |
beta-1,3-galactobiose | - |
Raphanus sativus | |
8.55 | - |
lactose | - |
Raphanus sativus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Raphanus sativus | - |
L. var. hortensis cv. Aokubi | - |
Purification (Comment) | Organism |
---|---|
- |
Raphanus sativus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
radicle | epidermal cells, intercellular space of cortical cells, endodermis surrounding a vascular bundle | Raphanus sativus | - |
seed | - |
Raphanus sativus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
5.62 | - |
- |
Raphanus sativus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylumbelliferyl-beta-D-galactoside + H2O | - |
Raphanus sativus | 4-methylumbelliferone + beta-D-galactose | - |
? | |
beta-1,3-galactobiose + H2O | - |
Raphanus sativus | beta-D-galactose | - |
? | |
beta-1,3-galactotriose + H2O | - |
Raphanus sativus | ? | - |
? | |
beta-1,6-galactobiose + H2O | - |
Raphanus sativus | beta-D-galactose | - |
? | |
beta-1,6-galactotriose + H2O | - |
Raphanus sativus | ? | - |
? | |
beta-D-Galp-(1->3)-beta-D-Galp-(1->3)-D-galactitol + H2O | - |
Raphanus sativus | beta-D-Galp-(1->3)-D-galactitol + D-galactopyranose | - |
? | |
beta-D-Galp-(1->6)-beta-D-Galp-(1->6)-D-galactitol + H2O | - |
Raphanus sativus | beta-D-Galp-(1->6)-D-galactitol + D-galactopyranose | - |
? | |
lactose + H2O | - |
Raphanus sativus | D-glucose + D-galactose | - |
? | |
methyl beta-D-galactohexaoside + H2O | beta-1,6-linked | Raphanus sativus | ? | - |
? | |
methyl beta-D-galactopentaoside + H2O | beta-1,3-linked or beta-1,6-linked | Raphanus sativus | ? | - |
? | |
methyl beta-D-galactotetraoside + H2O | beta-1,3-linked | Raphanus sativus | ? | - |
? | |
methyl O-beta-galactoside + H2O | - |
Raphanus sativus | methanol + beta-D-galactose | - |
? | |
additional information | specifically splits off beta-1,3-linked and beta-1,6-linked D-galactosyl residues from the nonreducing ends, their rates of hydrolysis increases with increasing chain length | Raphanus sativus | ? | - |
? | |
o-nitrophenyl-beta-D-galactoside + H2O | - |
Raphanus sativus | o-nitrophenol + beta-D-galactose | - |
? | |
p-nitrophenyl-beta-D-galactoside + H2O | - |
Raphanus sativus | p-nitrophenol + beta-D-galactose | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
pH 4.0, 10 min, stable up to | Raphanus sativus |
55 | - |
pH 4.0, 10 min, complete inactivation | Raphanus sativus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | - |
hydrolysis of p-nitrophenyl-beta-D-galactoside or beta-1,3-galactobiose | Raphanus sativus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3.5 | 8.5 | 4°C, 24 h, stable | Raphanus sativus |