Protein Variants | Comment | Organism |
---|---|---|
L317F | maximal velocity deviates from that of wild-type enzyme only at lower temperatures, in 2 mM Mg2+. This temperature-dependent effect can be suppressed by increasing the Mg2+ concentration | Lactobacillus delbrueckii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.365 | - |
o-nitrophenyl-beta-D-galactoside | wild-type enzyme, at 11-45°C | Lactobacillus delbrueckii | |
0.371 | - |
o-nitrophenyl-beta-D-galactoside | mutant enzyme L317F, 11-45°C | Lactobacillus delbrueckii | |
0.479 | - |
o-nitrophenyl-beta-D-galactoside | mutant enzyme P429S, at 11-45°C | Lactobacillus delbrueckii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
110000 | - |
2 * 110000, wild-type enzyme and mutant enzymes L317D and P429S, SDS-PAGE | Lactobacillus delbrueckii |
220000 | - |
gel filtration | Lactobacillus delbrueckii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lactobacillus delbrueckii | - |
subsp. bulgaricus, wild type enzyme and mutant enzymes P429S and L317F | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
o-nitrophenyl-beta-D-galactoside + H2O | - |
Lactobacillus delbrueckii | o-nitrophenol + beta-D-galactose | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 110000, wild-type enzyme and mutant enzymes L317D and P429S, SDS-PAGE | Lactobacillus delbrueckii |