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Literature summary for 3.2.1.23 extracted from

  • Adams, R.M.; Yoast, S.; Mainzer, S.E.; Moon, K.; Palombella, A.L.; Estell, D.A.; Power, S.D.; Schmidt, B.F.
    Characterization of two cold-sensitive mutants of the beta-galactosidase from Lactobacillus delbruckii subsp. Bulgaricus (1994), J. Biol. Chem., 8, 5666-5672.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
L317F maximal velocity deviates from that of wild-type enzyme only at lower temperatures, in 2 mM Mg2+. This temperature-dependent effect can be suppressed by increasing the Mg2+ concentration Lactobacillus delbrueckii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.365
-
o-nitrophenyl-beta-D-galactoside wild-type enzyme, at 11-45°C Lactobacillus delbrueckii
0.371
-
o-nitrophenyl-beta-D-galactoside mutant enzyme L317F, 11-45°C Lactobacillus delbrueckii
0.479
-
o-nitrophenyl-beta-D-galactoside mutant enzyme P429S, at 11-45°C Lactobacillus delbrueckii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
110000
-
2 * 110000, wild-type enzyme and mutant enzymes L317D and P429S, SDS-PAGE Lactobacillus delbrueckii
220000
-
gel filtration Lactobacillus delbrueckii

Organism

Organism UniProt Comment Textmining
Lactobacillus delbrueckii
-
subsp. bulgaricus, wild type enzyme and mutant enzymes P429S and L317F
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
o-nitrophenyl-beta-D-galactoside + H2O
-
Lactobacillus delbrueckii o-nitrophenol + beta-D-galactose
-
?

Subunits

Subunits Comment Organism
dimer 2 * 110000, wild-type enzyme and mutant enzymes L317D and P429S, SDS-PAGE Lactobacillus delbrueckii