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Literature summary for 3.2.1.22 extracted from
- Cloning and functional expression of an alpha-galactosidase from Yersinia pestis biovar Microtus str. 91001 (2008), Biosci. Biotechnol. Biochem., 72, 2203-2205.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Yersinia pestis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | inactivation | Yersinia pestis |  |
| Hg2+ | inactivation | Yersinia pestis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00135 | - |
melibiose | - |
Yersinia pestis | |
| 0.51 | - |
4-nitrophenyl alpha-D-galactoside | - |
Yersinia pestis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 80000 | - |
x * 80000, SDS-PAGE | Yersinia pestis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Yersinia pestis | - |
biovar Microtus str. 91001 | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 21.2 | - |
recombinant enzyme | Yersinia pestis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-methylumbelliferyl-alpha-D-galactoside + H2O | - |
Yersinia pestis | 4-methylumbelliferol + alpha-D-galactose | - |
? | |
| 4-nitrophenyl alpha-D-galactoside + H2O | - |
Yersinia pestis | 4-nitrophenol + alpha-D-galactose | - |
? | |
| melibiose + H2O | - |
Yersinia pestis | D-galactose + D-glucose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 80000, SDS-PAGE | Yersinia pestis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Aga-Y | - |
Yersinia pestis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Yersinia pestis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
10 min, 3% residual activity | Yersinia pestis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3279 | - |
4-nitrophenyl alpha-D-galactoside | - |
Yersinia pestis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | - |
- |
Yersinia pestis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
stable in narrow range around pH 7.0 | Yersinia pestis |
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