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Literature summary for 3.2.1.22 extracted from

  • Sinitsyna, O.A.; Fedorova, E.A.; Vakar, I.M.; Kondratieva, E.G.; Rozhkova, A.M.; Sokolova, L.M.; Bubnova, T.M.; Okunev, O.N.; Chulkin, A.M.; Vinetsky, Y.P.; Sinitsyn, A.P.
    Isolation and characterization of extracellular alpha-galactosidases from Penicillium canescens (2008), Biochemistry (Moscow), 73, 97-106.
    View publication on PubMed
Search for more literature for 3.2.1.22

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.22
-
 stachyose pH 5.0, 40°C Penicillium canescens
0.48
-
 4-nitrophenyl alpha-D-galactopyranoside pH 5.0, 40°C Penicillium canescens
0.49
-
 raffinose pH 5.0, 40°C Penicillium canescens
0.61
-
 4-nitrophenyl alpha-D-galactopyranoside pH 5.0, 40°C Penicillium canescens
2 3 raffinose pH 5.0, 40°C Penicillium canescens
21
-
 stachyose pH 5.0, 40°C Penicillium canescens
34
-
 galactomannan with galactose/mannose ratio 1:2.4 pH 5.0, 50°C Penicillium canescens
44
-
 galactomannan with galactose/mannose ratio 1:1.72 pH 5.0, 50°C Penicillium canescens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45856
-
 x * 61000, SDS-PAGE, x * 45856, calculated Penicillium canescens
61000
-
 x * 61000, SDS-PAGE, x * 45856, calculated Penicillium canescens
80000
-
 x * 80000, SDS-PAGE, x * 81858, calculated Penicillium canescens
81858
-
 x * 80000, SDS-PAGE, x * 81858, calculated Penicillium canescens

Organism

Organism UniProt Comment Textmining
Penicillium canescens
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5
-
 pH 5.0, 50°C, substrate galactomannan with galactose/mannose ratio 1:1.72 Penicillium canescens
6.2
-
 pH 5.0, 50°C, substrate galactomannan with galactose/mannose ratio 1:2.4 Penicillium canescens
98
-
 pH 5.0, 40°C, substrate 4-nitrophenyl-alpha-D-galactopyranoside Penicillium canescens
256
-
 pH 5.0, 50°C, substrate raffinose Penicillium canescens
256
-
 pH 5.0, 50°C, substrate stachyose Penicillium canescens
1255
-
 pH 5.0, 40°C, substrate 4-nitrophenyl-alpha-D-galactopyranoside Penicillium canescens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl alpha-D-galactopyranoside + H2O
-
 Penicillium canescens 4-nitrophenol + alpha-D-galactose
-
 ?
galactomannan + H2O with galactose/mannose ratios of 2.4 and of 1.72 Penicillium canescens ?
-
 ?
galactomannan with galactose/mannose ratio 1:1.72 + H2O
-
 Penicillium canescens ?
-
 ?
galactomannan with galactose/mannose ratio 1:2.4 + H2O
-
 Penicillium canescens ?
-
 ?
raffinose + H2O
-
 Penicillium canescens sucrose + D-galactose
-
 ?
raffinose + H2O
-
 Penicillium canescens sucrose + alpha-D-galactose
-
 ?
stachyose + H2O
-
 Penicillium canescens ?
-
 ?

Subunits

Subunits Comment Organism
? x * 61000, SDS-PAGE, x * 45856, calculated Penicillium canescens
? x * 80000, SDS-PAGE, x * 81858, calculated Penicillium canescens

Synonyms

Synonyms Comment Organism
AglA
-
 Penicillium canescens
AglC
-
 Penicillium canescens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 Penicillium canescens
65
-
-
 Penicillium canescens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.57
-
 stachyose pH 5.0, 40°C Penicillium canescens
0.89
-
 raffinose pH 5.0, 40°C Penicillium canescens
7
-
 4-nitrophenyl alpha-D-galactopyranoside pH 5.0, 40°C Penicillium canescens
7
-
 galactomannan with galactose/mannose ratio 1:1.72 pH 5.0, 50°C Penicillium canescens
10
-
 galactomannan with galactose/mannose ratio 1:2.4 pH 5.0, 50°C Penicillium canescens
1100
-
 raffinose pH 5.0, 40°C Penicillium canescens
1250
-
 stachyose pH 5.0, 40°C Penicillium canescens
1800
-
 4-nitrophenyl alpha-D-galactopyranoside pH 5.0, 40°C Penicillium canescens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4 5
-
 Penicillium canescens
5
-
-
 Penicillium canescens

pH Range

pH Minimum pH Maximum Comment Organism
2.5
-
 complete loss of activity Penicillium canescens
7
-
 complete loss of activity Penicillium canescens

pI Value

Organism Comment pI Value Maximum pI Value
Penicillium canescens isoelectric focusing
-
 4.8
Penicillium canescens calculated
-
 4.9
Penicillium canescens calculated
-
 5
Penicillium canescens isoelectric focusing
-
 5.1