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Literature summary for 3.2.1.22 extracted from

  • Goulas, T.; Goulas, A.; Tzortzis, G.; Gibson, G.R.
    A novel alpha-galactosidase from Bifidobacterium bifidum with transgalactosylating properties: gene molecular cloning and heterologous expression (2009), Appl. Microbiol. Biotechnol., 82, 471-477.
    View publication on PubMed
Search for more literature for 3.2.1.22

Cloned(Commentary)

Cloned (Comment) Organism
-
 Bifidobacterium bifidum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
82800
-
 3 * 82800, calculated, 3 * 85000, SDS-PAGE Bifidobacterium bifidum
85000
-
 3 * 82800, calculated, 3 * 85000, SDS-PAGE Bifidobacterium bifidum
243000
-
 gel filtration Bifidobacterium bifidum

Organism

Organism UniProt Comment Textmining
Bifidobacterium bifidum Q1KTD9
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl alpha-D-galactoside + H2O
-
 Bifidobacterium bifidum 4-nitrophenol + D-galactose
-
 ?
additional information under optimum pH conditions and high melibiose concentration of 40% w/v, the enzyme is able to form oligosaccharides with degree of polymerisation of 3 and more at higher concentration than with degree of polymerization 2, with a total yield of 20.5% w/w Bifidobacterium bifidum ?
-
 ?

Subunits

Subunits Comment Organism
trimer 3 * 82800, calculated, 3 * 85000, SDS-PAGE Bifidobacterium bifidum

Synonyms

Synonyms Comment Organism
MelA
-
 Bifidobacterium bifidum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Bifidobacterium bifidum