Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 107.8% activity at 10 mM | Aspergillus terreus | |
D-glucose | 105.1% activity at 10 mM | Aspergillus terreus | |
D-sucrose | 116.5% activity at 10 mM | Aspergillus terreus | |
EDTA | 105% activity at 10 mM | Aspergillus terreus | |
iodoacetamide | 104.3% activity at 10 mM | Aspergillus terreus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 98.66% activity at 10 mM | Glycine max | |
2-mercaptoethanol | 95% activity at 10 mM | Penicillium griseoroseum | |
Ag+ | complete inhibition at 10 mM | Aspergillus terreus | |
Ag+ | complete inhibition at 10 mM | Glycine max | |
Ag+ | complete inhibition at 10 mM | Penicillium griseoroseum | |
Ca2+ | 96.25% activity at 10 mM | Glycine max | |
Ca2+ | 94% activity at 10 mM | Penicillium griseoroseum | |
Cu2+ | complete inhibition at 10 mM | Aspergillus terreus | |
Cu2+ | 59.16% activity at 10 mM | Glycine max | |
Cu2+ | 11% activity at 10 mM | Penicillium griseoroseum | |
D-galactose | inhibitory effect at 10 mM | Glycine max | |
D-glucose | 94.4% activity at 10 mM | Glycine max | |
D-glucose | 96% activity at 10 mM | Penicillium griseoroseum | |
D-lactose | 89.51% activity at 10 mM | Glycine max | |
D-lactose | 88% activity at 10 mM | Penicillium griseoroseum | |
D-maltose | 92.8% activity at 10 mM | Aspergillus terreus | |
D-maltose | 91.33% activity at 10 mM | Glycine max | |
D-maltose | 94% activity at 10 mM | Penicillium griseoroseum | |
D-mannose | 90.5% activity at 10 mM | Aspergillus terreus | |
D-mannose | 90.05% activity at 10 mM | Glycine max | |
D-mannose | 94% activity at 10 mM | Penicillium griseoroseum | |
D-sucrose | 98.72% activity at 10 mM | Glycine max | |
EDTA | 97.21% activity at 10 mM | Glycine max | |
Hg2+ | complete inhibition at 10 mM | Aspergillus terreus | |
Hg2+ | complete inhibition at 10 mM | Glycine max | |
Hg2+ | complete inhibition at 10 mM | Penicillium griseoroseum | |
iodoacetamide | 95.88% activity at 10 mM | Glycine max | |
iodoacetamide | 98.87% activity at 10 mM | Penicillium griseoroseum | |
K+ | 96.48% activity at 10 mM | Glycine max | |
melibiose | 39.7% activity at 10 mM | Aspergillus terreus | |
melibiose | 74.14% activity at 10 mM | Glycine max | |
melibiose | 58% activity at 10 mM | Penicillium griseoroseum | |
Mg2+ | 94.15% activity at 10 mM | Glycine max | |
Mg2+ | 98% activity at 10 mM | Penicillium griseoroseum | |
additional information | not inhibited by D-lactose | Aspergillus terreus | |
additional information | not inhibited by sucrose, EDTA, and K+ | Penicillium griseoroseum | |
Na+ | 94.2% activity at 10 mM | Glycine max | |
Na+ | 96% activity at 10 mM | Penicillium griseoroseum | |
raffinose | 80.60% activity at 10 mM | Aspergillus terreus | |
raffinose | 81.73% activity at 10 mM | Glycine max | |
raffinose | 93% activity at 10 mM | Penicillium griseoroseum | |
SDS | 46.9% activity at 10 mM | Aspergillus terreus | |
SDS | 49.36% activity at 10 mM | Glycine max | |
SDS | complete inhibition at 10 mM | Penicillium griseoroseum | |
stachyose | 95.73% activity at 10 mM | Aspergillus terreus | |
stachyose | 87.69% activity at 10 mM | Glycine max | |
stachyose | 88% activity at 10 mM | Penicillium griseoroseum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 109.9% activity at 10 mM | Aspergillus terreus | |
K+ | 105.7% activity at 10 mM | Aspergillus terreus | |
Mg2+ | 105.6% activity at 10 mM | Aspergillus terreus | |
Na+ | 124.8% activity at 10 mM | Aspergillus terreus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus terreus | - |
- |
- |
Glycine max | - |
cultivar Monarca | - |
Penicillium griseoroseum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate fractionation, Sephacryl S-200 gel filtration | Penicillium griseoroseum |
ammonium sulfate fractionation, Sephadex G-100 gel filtration, CM-Sepharose Fast Flow column chromatography | Glycine max |
Sephacryl S-200 gel filtration | Aspergillus terreus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seed | - |
Glycine max | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.02 | - |
crude extract, in 0.1 M sodium acetate buffer, pH 5.0, at 40°C | Penicillium griseoroseum |
3.33 | - |
crude extract, in 0.1 M sodium acetate buffer, pH 4.0, at 40°C | Aspergillus terreus |
30 | - |
crude extract, in 0.1 M sodium acetate buffer, pH 5.0, at 40°C | Glycine max |
55 | - |
after 27.2fold purification, in 0.1 M sodium acetate buffer, pH 5.0, at 40°C | Penicillium griseoroseum |
67.3 | - |
after 20.20fold purification, in 0.1 M sodium acetate buffer, pH 4.0, at 40°C | Aspergillus terreus |
540 | - |
after 19.74fold purification, in 0.1 M sodium acetate buffer, pH 5.0, at 40°C | Glycine max |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl alpha-D-glucopyranoside + H2O | - |
Penicillium griseoroseum | 4-nitrophenol + D-glucose | - |
? | |
4-nitrophenyl alpha-D-xylopyranoside + H2O | 9.64% activity relative to p-nitrophenyl alpha-D-galactopyranoside | Aspergillus terreus | 4-nitrophenol + alpha-D-xylopyranose | - |
? | |
D-sucrose + H2O | 16.30% activity relative to p-nitrophenyl alpha-D-galactopyranoside | Penicillium griseoroseum | D-glucose + D-fructose | - |
? | |
D-sucrose + H2O | 35.71% activity relative to p-nitrophenyl alpha-D-galactopyranoside | Aspergillus terreus | D-glucose + D-fructose | - |
? | |
lactose + H2O | 2.3% activity relative to p-nitrophenyl alpha-D-galactopyranoside | Glycine max | beta-D-galactose + D-glucose | - |
? | |
maltose + H2O | 10.60% activity relative to p-nitrophenyl alpha-D-galactopyranoside | Penicillium griseoroseum | alpha-D-glucose + D-glucose | - |
? | |
maltose + H2O | 18.12% activity relative to p-nitrophenyl alpha-D-galactopyranoside | Glycine max | alpha-D-glucose + D-glucose | - |
? | |
maltose + H2O | 6.07% activity relative to p-nitrophenyl alpha-D-galactopyranoside | Aspergillus terreus | alpha-D-glucose + D-glucose | - |
? | |
melibiose + H2O | 15.36% activity relative to p-nitrophenyl alpha-D-galactopyranoside | Aspergillus terreus | D-galactose + D-glucose | - |
? | |
melibiose + H2O | 16.15% activity relative to p-nitrophenyl alpha-D-galactopyranoside | Penicillium griseoroseum | D-galactose + D-glucose | - |
? | |
melibiose + H2O | 7.68% activity relative to p-nitrophenyl alpha-D-galactopyranoside | Glycine max | D-galactose + D-glucose | - |
? | |
additional information | D-lactose, o-nitrophenyl beta-D-galactopyranoside, p-nitrophenyl alpha-D-glucopyranoside, p-nitrophenyl alpha-D-mannopyranoside, and p-nitrophenyl beta-D-galactopyranoside are no substrates | Aspergillus terreus | ? | - |
? | |
additional information | D-sucrose, p-nitrophenyl alpha-D-glucopyranoside, p-nitrophenyl alpha-D-xylulopyranoside, p-nitrophenyl alpha-D-mannopyranoside, and p-nitrophenyl beta-D-galactopyranoside are no substrates | Glycine max | ? | - |
? | |
additional information | lactose, o-nitrophenyl beta-D-galactopyranoside, p-nitrophenyl alpha-D-xylulopyranoside, p-nitrophenyl alpha-D-mannopyranoside, and p-nitrophenyl beta-D-galactopyranoside are no substrates | Penicillium griseoroseum | ? | - |
? | |
o-nitrophenyl beta-D-galactopyranoside | 4.53% activity relative to p-nitrophenyl alpha-D-galactopyranoside | Glycine max | o-nitrophenol + beta-D-galactopyranose | - |
? | |
p-nitrophenyl alpha-D-galactopyranoside + H2O | 100% activity | Glycine max | p-nitrophenol + alpha-D-galactose | - |
? | |
p-nitrophenyl alpha-D-galactopyranoside + H2O | 100% activity | Aspergillus terreus | p-nitrophenol + alpha-D-galactose | - |
? | |
p-nitrophenyl alpha-D-galactopyranoside + H2O | 100% activity | Penicillium griseoroseum | p-nitrophenol + alpha-D-galactose | - |
? | |
raffinose + H2O | 30.60% activity relative to p-nitrophenyl alpha-D-galactopyranoside | Glycine max | sucrose + D-galactose | - |
? | |
raffinose + H2O | 60.65% activity relative to p-nitrophenyl alpha-D-galactopyranoside | Penicillium griseoroseum | sucrose + D-galactose | - |
? | |
raffinose + H2O | 78.57% activity relative to p-nitrophenyl alpha-D-galactopyranoside | Aspergillus terreus | sucrose + D-galactose | - |
? | |
stachyose + 2 H2O | 11.74% activity relative to p-nitrophenyl alpha-D-galactopyranoside | Glycine max | 2 alpha-D-galactose + sucrose | - |
? | |
stachyose + 2 H2O | 78.65% activity relative to p-nitrophenyl alpha-D-galactopyranoside | Penicillium griseoroseum | 2 alpha-D-galactose + sucrose | - |
? | |
stachyose + 2 H2O | 85.71% activity relative to p-nitrophenyl alpha-D-galactopyranoside | Aspergillus terreus | 2 alpha-D-galactose + sucrose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Alpha-D-galactoside galactohydrolase | - |
Glycine max |
Alpha-D-galactoside galactohydrolase | - |
Aspergillus terreus |
Alpha-D-galactoside galactohydrolase | - |
Penicillium griseoroseum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Penicillium griseoroseum |
50 | - |
- |
Glycine max |
65 | - |
- |
Aspergillus terreus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 50 | no loss in enzyme activity is observed after incubation for 5 h at 40°C, the enzyme maintains about 65% of its original activity after 2 h at 45°C, about 90% of the activity is lost during 30 min at 50°C | Penicillium griseoroseum |
40 | 50 | the enzyme maintains about 90% and 70% of its original activity after 4 h and 10 h at 40°C, respectively, at 50°C 91% of its original activity is lost after 30 min of incubation | Glycine max |
60 | - |
the enzyme maintains about 100% and 40% of its original activity after 3 h and 36 h at 60°C, respectively | Aspergillus terreus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | - |
- |
Aspergillus terreus |
5 | - |
- |
Glycine max |
5 | - |
- |
Penicillium griseoroseum |