Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain Origami (DE) | Oryza sativa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | alpha-Gal II, 67% decrease of activity at 1 mM; alpha-Gal III, 84% decrease of activity at 1 mM | Oryza sativa | |
Hg2+ | alpha-Gal II, 93% decrease of activity at 1 mM; alpha-Gal III, 87% decrease of activity at 1 mM | Oryza sativa | |
p-chloromercuribenzoate | alpha-Gal III shows less than 8% of the original activity by treatment with 0.1 mM p-chloromercuribenzoate at 20°C for 1 h; alpha-Gal II shows less than 6% of the original activity by treatment with 0.1 mM p-chloromercuribenzoate at 20°C for 1 h | Oryza sativa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | Ca2+, Cu2+, Fe2+, Mg2+, Mn2+, Ni2+, and Zn2+ do not affect activity | Oryza sativa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
41020 | - |
calculated from amino acid sequence | Oryza sativa |
41520 | - |
calculated from amino acid sequence | Oryza sativa |
42000 | - |
SDS-PAGE | Oryza sativa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryza sativa | Q7XIV4 | variant Nipponbare | - |
Oryza sativa | Q9FXT4 | alpha-Gal II; variant Nipponbare | - |
Purification (Comment) | Organism |
---|---|
Ni2+-Sepharose column chromatography | Oryza sativa |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.31 | - |
alpha-Gal III, crude extract | Oryza sativa |
2.25 | - |
alpha-Gal II, crude extract | Oryza sativa |
9.13 | - |
alpha-Gal III, after 29.5fold purification | Oryza sativa |
52.8 | - |
alpha-Gal II, after 23.5fold purification | Oryza sativa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
6(1)-alpha-D-galactosyl mannobiose + H2O | 2.6% relative activity compared to p-nitrophenyl alpha-D-galactoside | Oryza sativa | ? | - |
? | |
6(1)-alpha-D-galactosyl mannobiose + H2O | 6.8% relative activity compared to p-nitrophenyl alpha-D-galactoside | Oryza sativa | ? | - |
? | |
6(1)-alpha-D-galactosyl mannotriose + H2O | 2.7% relative activity compared to p-nitrophenyl alpha-D-galactoside | Oryza sativa | ? | - |
? | |
6(1)-alpha-D-galactosyl mannotriose + H2O | 6.9% relative activity compared to p-nitrophenyl alpha-D-galactoside | Oryza sativa | ? | - |
? | |
6(3),6(4)-di-alpha-D-galactosyl mannopentaose + H2O | 0.5% relative activity compared to p-nitrophenyl alpha-D-galactoside | Oryza sativa | ? | - |
? | |
6(3),6(4)-di-alpha-D-galactosyl mannopentaose + H2O | 7.3% relative activity compared to p-nitrophenyl alpha-D-galactoside | Oryza sativa | ? | - |
? | |
guar gum + H2O | 0.4% relative activity compared to p-nitrophenyl alpha-D-galactoside | Oryza sativa | ? | - |
? | |
guar gum + H2O | 1.7% relative activity compared to p-nitrophenyl alpha-D-galactoside | Oryza sativa | ? | - |
? | |
locust bean gum + H2O | 0.4% relative activity compared to p-nitrophenyl alpha-D-galactoside | Oryza sativa | ? | - |
? | |
locust bean gum + H2O | 1.4% relative activity compared to p-nitrophenyl alpha-D-galactoside | Oryza sativa | ? | - |
? | |
melibiose + H2O | 1.2% relative activity compared to p-nitrophenyl alpha-D-galactoside | Oryza sativa | D-galactose + D-glucose | - |
? | |
melibiose + H2O | 6.9% relative activity compared to p-nitrophenyl alpha-D-galactoside | Oryza sativa | D-galactose + D-glucose | - |
? | |
p-nitrophenyl alpha-D-galactoside + H2O | 100% activity | Oryza sativa | p-nitrophenol + alpha-D-galactose | - |
? | |
raffinose + H2O | 1.89% relative activity compared to p-nitrophenyl alpha-D-galactoside | Oryza sativa | sucrose + alpha-D-galactose | - |
? | |
raffinose + H2O | 10.1% relative activity compared to p-nitrophenyl alpha-D-galactoside | Oryza sativa | sucrose + alpha-D-galactose | - |
? | |
stachyose + 2 H2O | 0.47% relative activity compared to p-nitrophenyl alpha-D-galactoside | Oryza sativa | 2 alpha-D-galactose + sucrose | - |
? | |
stachyose + 2 H2O | 1.9% relative activity compared to p-nitrophenyl alpha-D-galactoside | Oryza sativa | 2 alpha-D-galactose + sucrose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
alpha-Gal II | - |
Oryza sativa |
alpha-Gal III | - |
Oryza sativa |
alpha-galactosidase II | - |
Oryza sativa |
alpha-galactosidase III | - |
Oryza sativa |
alpha-galactoside galactohydrolase | - |
Oryza sativa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Oryza sativa |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
alpha-Gal II is stable for 1 h up to 35°C at pH 5.0 | Oryza sativa |
35 | - |
alpha-Gal III is stable for 1 h up to 20°C at pH 5.0 | Oryza sativa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Oryza sativa |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3.5 | 8.5 | alpha-Gal II is stable at pH 3.5-8.5 at 25°C | Oryza sativa |
4 | 7 | alpha-Gal III is stable at pH 4.0-7.0 at 20°C | Oryza sativa |