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Literature summary for 3.2.1.22 extracted from

  • Rezessy-Szabo, J.M.; Nguyen, Q.D.; Hoschke, A.; Braet, C.; Hajos, G.; Claeyssens, M.
    A novel thermostable alpha -galactosidase from the thermophilic fungus Thermomyces lanuginosus CBS 395.62/b: Purification and characterization (2007), Biochim. Biophys. Acta, 1770, 55-62.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ strong inhibition at 10 mM Thermomyces lanuginosus
Co2+ slight inhibition at 10 mM Thermomyces lanuginosus
Cu2+ slight inhibition at 10 mM Thermomyces lanuginosus
Hg+ complete inactivation at 10 mM Thermomyces lanuginosus
Ni2+ slight inhibition at 10 mM Thermomyces lanuginosus
Zn2+ strong inhibition at 10 mM Thermomyces lanuginosus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13
-
p-nitrophenyl alpha-D-galactopyranoside at 55°C, in 50 mM citrate buffer (pH 4.2) Thermomyces lanuginosus
1.17
-
stachyose at 55°C, in 50 mM citrate buffer (pH 4.2) Thermomyces lanuginosus
1.61
-
raffinose at 55°C, in 50 mM citrate buffer (pH 4.2) Thermomyces lanuginosus

Metals/Ions

Metals/Ions Comment Organism Structure
K+ activates at 10 mM Thermomyces lanuginosus
Mg2+ activates at 10 mM Thermomyces lanuginosus
Mn2+ activates at 10 mM Thermomyces lanuginosus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
93000
-
native enzyme, SDS-PAGE Thermomyces lanuginosus

Organism

Organism UniProt Comment Textmining
Thermomyces lanuginosus
-
-
-
Thermomyces lanuginosus CBS 395.62/b
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein the total carbohydrate content of the purified enzyme is 5.3% Thermomyces lanuginosus

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate fractionation, Sepharose CL-6B column chromatography, DEAE-Sepharose FF column chromatography, and Superose 12 gel filtration Thermomyces lanuginosus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
15.9
-
unpurified enzyme from fermentation broth, using p-nitrophenyl alpha-D-galactopyranoside as a substrate Thermomyces lanuginosus
1813
-
after 114fold purification, using p-nitrophenyl alpha-D-galactopyranoside as a substrate Thermomyces lanuginosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
p-nitrophenyl alpha-D-galactopyranoside + H2O
-
Thermomyces lanuginosus p-nitrophenol + alpha-D-galactose
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
-
Thermomyces lanuginosus CBS 395.62/b p-nitrophenol + alpha-D-galactose
-
?
raffinose + H2O
-
Thermomyces lanuginosus sucrose + D-galactose
-
?
raffinose + H2O
-
Thermomyces lanuginosus CBS 395.62/b sucrose + D-galactose
-
?
stachyose + 2 H2O
-
Thermomyces lanuginosus 2 alpha-D-galactose + sucrose
-
?
stachyose + 2 H2O
-
Thermomyces lanuginosus CBS 395.62/b 2 alpha-D-galactose + sucrose
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
-
Thermomyces lanuginosus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
retains more than 90% of the initial activity at 45°C in a pH range from 5.5-9.0 Thermomyces lanuginosus
65
-
5 min incubation at 65°C results in complete inactivation of the purified enzyme Thermomyces lanuginosus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3914
-
p-nitrophenyl alpha-D-galactopyranoside at 55°C, in 50 mM citrate buffer (pH 4.2) Thermomyces lanuginosus
6946
-
raffinose at 55°C, in 50 mM citrate buffer (pH 4.2) Thermomyces lanuginosus
7659
-
stachyose at 55°C, in 50 mM citrate buffer (pH 4.2) Thermomyces lanuginosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5 5.5
-
Thermomyces lanuginosus

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5 9 retains more than 90% of the initial activity at 45°C in a pH range from 5.5-9.0 Thermomyces lanuginosus

pI Value

Organism Comment pI Value Maximum pI Value
Thermomyces lanuginosus isoelectric focusing
-
3.9