Cloned (Comment) | Organism |
---|---|
overexpression in Sf9 cells infected with recombinant baculovirus, expression in COS-7 cells | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
efficient and rapid purification procedure for recombinant alpha-galactosidase A by sequential Concanavalin A-Sepharose and immobilized thio-alpha-galactoside agarose column chromatography. This procedure is especially useful for the purification of mutant forms of alpha-galactosidase A, which are not stable under conventional purification techniques. Purification of the intracellular mutant enzyme M279I, expressed in COS-7 cells within 6 h at 62% overall yield | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylumbelliferyl alpha-D-galactoside + H2O | - |
Homo sapiens | alpha-D-galactose + 4-methylumbelliferone | - |
? |