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Literature summary for 3.2.1.22 extracted from

  • Kusumoto, K.; Shirahata, S.; Kamei, Y.
    Purification and characterization of alpha-D-galactosidase produced by ADG cell line established from abalon digestive gland (2000), Cytotechnology, 33, 47-52.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.22

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
 gel filtration Haliotis corrugata
43000
-
 1 * 43000, SDS-PAGE Haliotis corrugata

Organism

Organism UniProt Comment Textmining
Haliotis corrugata
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Haliotis corrugata

Source Tissue

Source Tissue Comment Organism Textmining
ADG cell line abalone digestive gland Haliotis corrugata
-
ADG cell line ADG cell line Haliotis corrugata
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
p-nitrophenyl alpha-D-galactopyranoside + H2O
-
 Haliotis corrugata p-nitrophenol + alpha-D-galactose
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 43000, SDS-PAGE Haliotis corrugata

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Haliotis corrugata

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 50 25°C: about 35% of maximal activity, 50°C: 40% of maximal activity Haliotis corrugata

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 Haliotis corrugata

pH Range

pH Minimum pH Maximum Comment Organism
4.5 7 pH 4.0: about 15% of maximal activity, pH 4.5: about 90% of maximal activity, pH 7.0: about 45% of maximal activity Haliotis corrugata

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5
-
 4°C, 24 h, most stable at Haliotis corrugata