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Literature summary for 3.2.1.22 extracted from
- Isozymes of alpha-Galactosidas from Bacillus stearothermophilus (1980), Can. J. Microbiol., 26, 978-984.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.23 | - |
p-nitrophenyl-alpha-D-galactopyranoside | alpha-galactosidase II, pH 6 | Geobacillus stearothermophilus |  |
| 0.47 | - |
p-nitrophenyl-alpha-D-galactopyranoside | alpha-galactosidase I, pH 7 | Geobacillus stearothermophilus | |
| 0.53 | - |
p-nitrophenyl-alpha-D-galactopyranoside | alpha-galactosidase II, pH 7 | Geobacillus stearothermophilus | |
| 0.59 | - |
p-nitrophenyl-alpha-D-galactopyranoside | alpha-galactosidase I, pH 6 | Geobacillus stearothermophilus | |
| 19 | - |
melibiose | alpha-galactosidase I | Geobacillus stearothermophilus | |
| 45 | - |
melibiose | alpha-galactosidase II | Geobacillus stearothermophilus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 280000 | - |
disc gel electrophoresis, alpha-galactosidase I | Geobacillus stearothermophilus |
| 325000 | - |
disc gel electrophoresis, alpha-galactosidase II | Geobacillus stearothermophilus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Geobacillus stearothermophilus | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 217 | - |
alpha-galactosidase II | Geobacillus stearothermophilus |
| 290 | - |
alpha-galactosidase I | Geobacillus stearothermophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| melibiose + H2O | - |
Geobacillus stearothermophilus | D-galactose + D-glucose | - |
? | |
| p-nitrophenyl-alpha-D-galactoside + H2O | - |
Geobacillus stearothermophilus | p-nitrophenol + alpha-D-galactose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | - |
Geobacillus stearothermophilus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
alpha-galactosidase I | Geobacillus stearothermophilus |
| 7 | - |
alpha-galactosidase II | Geobacillus stearothermophilus |
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Release 2023.1 (January 2023)
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