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Literature summary for 3.2.1.22 extracted from
- Tomato alpha-Galactosidases: conversion of human type B erythrocytes to type O (1984), Phytochemistry, 23, 55-58.
No PubMed abstract available
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.22 | - |
p-nitrophenyl alpha-D-galactoside | alpha-galactosidase II | Solanum lycopersicum |  |
| 0.54 | - |
p-nitrophenyl alpha-D-galactoside | alpha-galactosidase I | Solanum lycopersicum | |
| 1.8 | - |
raffinose | alpha-galactosidase I | Solanum lycopersicum | |
| 2.4 | - |
stachyose | alpha-galactosidase I | Solanum lycopersicum | |
| 2.7 | - |
raffinose | alpha-galactosidase II | Solanum lycopersicum | |
| 3.8 | - |
stachyose | alpha-galactosidase II | Solanum lycopersicum | |
| 5.3 | - |
methyl alpha-D-galactoside | alpha-galactosidase I | Solanum lycopersicum | |
| 8.4 | - |
methyl alpha-D-galactoside | alpha-galactosidase II | Solanum lycopersicum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 19000 | - |
alpha-galactosidase II, gel filtration | Solanum lycopersicum |
| 44000 | - |
alpha-galactosidase I, gel filtration | Solanum lycopersicum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Solanum lycopersicum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Solanum lycopersicum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.3 | - |
alpha-galactosidase I | Solanum lycopersicum |
| 82 | - |
alpha-galactosidase II | Solanum lycopersicum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| galactomannan + H2O | - |
Solanum lycopersicum | ? | - |
? | |
| methyl alpha-D-galactoside + H2O | - |
Solanum lycopersicum | methanol + D-galactose | - |
? | |
| p-nitrophenyl-alpha-D-galactoside + H2O | - |
Solanum lycopersicum | p-nitrophenol + alpha-D-galactose | - |
? | |
| raffinose + H2O | - |
Solanum lycopersicum | sucrose + D-galactose | - |
? | |
| stachyose + H2O | - |
Solanum lycopersicum | ? | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
5 min, pH 4.5, 50% inactivation, alpha-galactosidase II | Solanum lycopersicum |
| 70 | - |
5 min, pH 5.2, 50% inactivation, alpha-galactosidase I | Solanum lycopersicum |
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Release 2023.1 (January 2023)
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