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Literature summary for 3.2.1.21 extracted from

  • Zanoelo, F.F.; Polizeli, M.d.e. .L.; Terenzi, H.F.; Jorge, J.A.
    Beta-glucosidase activity from the thermophilic fungus Scytalidium thermophilum is stimulated by glucose and xylose (2004), FEMS Microbiol. Lett., 240, 137-143 .
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
D-galactose 50 mM, 123% of initial activity Mycothermus thermophilus
D-glucose 50 mM, 204% of initial activity, activating constant 36.7 mM Mycothermus thermophilus
D-xylose 50 mM, 191% of initial activity, activating constant 43.2 mM Mycothermus thermophilus
additional information mannose, fructose, ribose, galactose, sorbose, arabinose, maltose and sucrose are without effect Mycothermus thermophilus

Inhibitors

Inhibitors Comment Organism Structure
cellobiose 50 mM, 24% residual activity Mycothermus thermophilus
additional information mannose, fructose, ribose, galactose, sorbose, arabinose, maltose and sucrose are without effect Mycothermus thermophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.29
-
4-nitrophenyl beta-D-glucopyranoside pH 6.5, 60°C Mycothermus thermophilus
1.26
-
4-nitrophenyl beta-D-glucopyranoside presence of D-glucose, pH 6.5, 60°C Mycothermus thermophilus
1.33
-
4-nitrophenyl beta-D-glucopyranoside presence of D-xylose, pH 6.5, 60°C Mycothermus thermophilus
1.61
-
cellobiose pH 6.5, 60°C Mycothermus thermophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39000
-
gel filtration Mycothermus thermophilus

Organism

Organism UniProt Comment Textmining
Mycothermus thermophilus
-
-
-
Mycothermus thermophilus CBS 619.91
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein 15% carbohydrate content Mycothermus thermophilus

Purification (Commentary)

Purification (Comment) Organism
-
Mycothermus thermophilus

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
Mycothermus thermophilus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.7
-
substrate 4-nitrophenyl alpha-D-arabinopyranoside, pH 6.5, 60°C Mycothermus thermophilus
0.9
-
substrate lactose, pH 6.5, 60°C Mycothermus thermophilus
1.4
-
substrate 4-nitrophenyl beta-D-xylopyranoside, pH 6.5, 60°C Mycothermus thermophilus
1.6
-
substrate laminaribiose, pH 6.5, 60°C Mycothermus thermophilus
1.8
-
substrate 4-nitrophenyl beta-D-galactopyranoside, pH 6.5, 60°C Mycothermus thermophilus
3.8
-
substrate cellobiose, pH 6.5, 60°C Mycothermus thermophilus
9.1
-
substrate 4-nitrophenyl beta-D-glucopyranoside, pH 6.5, 60°C Mycothermus thermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl alpha-L-arabinofuranoside + H2O
-
Mycothermus thermophilus 4-nitrophenol + alpha-L-arabinofuranose
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
-
Mycothermus thermophilus CBS 619.91 4-nitrophenol + alpha-L-arabinofuranose
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
-
Mycothermus thermophilus 4-nitrophenol + beta-D-galactopyranose
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
-
Mycothermus thermophilus CBS 619.91 4-nitrophenol + beta-D-galactopyranose
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
Mycothermus thermophilus 4-nitrophenol + D-glucose
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
Mycothermus thermophilus CBS 619.91 4-nitrophenol + D-glucose
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
-
Mycothermus thermophilus 4-nitrophenol + D-xylose
-
?
cellobiose + H2O
-
Mycothermus thermophilus 2 D-glucose
-
?
cellobiose + H2O
-
Mycothermus thermophilus CBS 619.91 2 D-glucose
-
?
lactose + H2O
-
Mycothermus thermophilus D-glucose + D-galactose
-
?
lactose + H2O
-
Mycothermus thermophilus CBS 619.91 D-glucose + D-galactose
-
?
laminaribiose + H2O
-
Mycothermus thermophilus 2 D-glucose
-
?

Subunits

Subunits Comment Organism
monomer 1 * 42000, SDS-PAGE Mycothermus thermophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
Mycothermus thermophilus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
55
-
90% of maximum activity Mycothermus thermophilus
65
-
10% of maximum activity Mycothermus thermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
stable up to 1 h Mycothermus thermophilus
55
-
half-life 20 min Mycothermus thermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
Mycothermus thermophilus

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 8 25°C, stable up to 1 h Mycothermus thermophilus

pI Value

Organism Comment pI Value Maximum pI Value
Mycothermus thermophilus isoelectric focusing
-
6.5

Expression

Organism Comment Expression
Mycothermus thermophilus addition of glucose to avicel or cellobiose culture media drastically represses the production of enzyme down