Activating Compound | Comment | Organism | Structure |
---|---|---|---|
D-galactose | 50 mM, 123% of initial activity | Mycothermus thermophilus | |
D-glucose | 50 mM, 204% of initial activity, activating constant 36.7 mM | Mycothermus thermophilus | |
D-xylose | 50 mM, 191% of initial activity, activating constant 43.2 mM | Mycothermus thermophilus | |
additional information | mannose, fructose, ribose, galactose, sorbose, arabinose, maltose and sucrose are without effect | Mycothermus thermophilus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
cellobiose | 50 mM, 24% residual activity | Mycothermus thermophilus | |
additional information | mannose, fructose, ribose, galactose, sorbose, arabinose, maltose and sucrose are without effect | Mycothermus thermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.29 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 6.5, 60°C | Mycothermus thermophilus | |
1.26 | - |
4-nitrophenyl beta-D-glucopyranoside | presence of D-glucose, pH 6.5, 60°C | Mycothermus thermophilus | |
1.33 | - |
4-nitrophenyl beta-D-glucopyranoside | presence of D-xylose, pH 6.5, 60°C | Mycothermus thermophilus | |
1.61 | - |
cellobiose | pH 6.5, 60°C | Mycothermus thermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39000 | - |
gel filtration | Mycothermus thermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycothermus thermophilus | - |
- |
- |
Mycothermus thermophilus CBS 619.91 | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | 15% carbohydrate content | Mycothermus thermophilus |
Purification (Comment) | Organism |
---|---|
- |
Mycothermus thermophilus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | - |
Mycothermus thermophilus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.7 | - |
substrate 4-nitrophenyl alpha-D-arabinopyranoside, pH 6.5, 60°C | Mycothermus thermophilus |
0.9 | - |
substrate lactose, pH 6.5, 60°C | Mycothermus thermophilus |
1.4 | - |
substrate 4-nitrophenyl beta-D-xylopyranoside, pH 6.5, 60°C | Mycothermus thermophilus |
1.6 | - |
substrate laminaribiose, pH 6.5, 60°C | Mycothermus thermophilus |
1.8 | - |
substrate 4-nitrophenyl beta-D-galactopyranoside, pH 6.5, 60°C | Mycothermus thermophilus |
3.8 | - |
substrate cellobiose, pH 6.5, 60°C | Mycothermus thermophilus |
9.1 | - |
substrate 4-nitrophenyl beta-D-glucopyranoside, pH 6.5, 60°C | Mycothermus thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl alpha-L-arabinofuranoside + H2O | - |
Mycothermus thermophilus | 4-nitrophenol + alpha-L-arabinofuranose | - |
? | |
4-nitrophenyl alpha-L-arabinofuranoside + H2O | - |
Mycothermus thermophilus CBS 619.91 | 4-nitrophenol + alpha-L-arabinofuranose | - |
? | |
4-nitrophenyl beta-D-galactopyranoside + H2O | - |
Mycothermus thermophilus | 4-nitrophenol + beta-D-galactopyranose | - |
? | |
4-nitrophenyl beta-D-galactopyranoside + H2O | - |
Mycothermus thermophilus CBS 619.91 | 4-nitrophenol + beta-D-galactopyranose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Mycothermus thermophilus | 4-nitrophenol + D-glucose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Mycothermus thermophilus CBS 619.91 | 4-nitrophenol + D-glucose | - |
? | |
4-nitrophenyl beta-D-xylopyranoside + H2O | - |
Mycothermus thermophilus | 4-nitrophenol + D-xylose | - |
? | |
cellobiose + H2O | - |
Mycothermus thermophilus | 2 D-glucose | - |
? | |
cellobiose + H2O | - |
Mycothermus thermophilus CBS 619.91 | 2 D-glucose | - |
? | |
lactose + H2O | - |
Mycothermus thermophilus | D-glucose + D-galactose | - |
? | |
lactose + H2O | - |
Mycothermus thermophilus CBS 619.91 | D-glucose + D-galactose | - |
? | |
laminaribiose + H2O | - |
Mycothermus thermophilus | 2 D-glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 42000, SDS-PAGE | Mycothermus thermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Mycothermus thermophilus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
90% of maximum activity | Mycothermus thermophilus |
65 | - |
10% of maximum activity | Mycothermus thermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
stable up to 1 h | Mycothermus thermophilus |
55 | - |
half-life 20 min | Mycothermus thermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Mycothermus thermophilus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 8 | 25°C, stable up to 1 h | Mycothermus thermophilus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Mycothermus thermophilus | isoelectric focusing | - |
6.5 |
Organism | Comment | Expression |
---|---|---|
Mycothermus thermophilus | addition of glucose to avicel or cellobiose culture media drastically represses the production of enzyme | down |