Literature summary for 3.2.1.21 extracted from

Huang, Y.; Busk, P.; Grell, M.; Zhao, H.; Lange, L.
Identification of a beta-glucosidase from the Mucor circinelloides genome by peptide pattern recognition (2014), Enzyme Microb. Technol., 67, 47-52 .

Search for more literature for 3.2.1.21

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Pichia pastoris	Mucor circinelloides

Crystallization (Commentary)

Crystallization (Comment)	Organism
modeling of structure	Mucor circinelloides

Inhibitors

Inhibitors	Comment	Organism	Structure
additional information	not inhibitory: D-glucose, enzyme retains 84% activity at glucose concentrations up to 140 mM	Mucor circinelloides

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.2	-	4-nitrophenyl beta-D-glucopyranoside	pH 5, 50°C	Mucor circinelloides

Organism

Organism	UniProt	Comment	Textmining
Mucor circinelloides	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-methylumbelliferyl beta-D-glucopyranoside + H2O	-	Mucor circinelloides	4-methylumbelliferone + beta-D-glucose	-	?
4-nitrophenyl beta-D-glucopyranoside + H2O	-	Mucor circinelloides	4-nitrophenol + D-glucose	-	?

Subunits

Subunits	Comment	Organism
?	x * 78240, calculated, x * 75000, SDS-PAGE	Mucor circinelloides

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
50	-	-	Mucor circinelloides

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
40	-	1 h, 58% residual activity	Mucor circinelloides

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
5	-	-	Mucor circinelloides

pI Value

Organism	Comment	pI Value Maximum	pI Value
Mucor circinelloides	calculated	-	5.6

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Release 2023.1 (January 2023)