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Literature summary for 3.2.1.21 extracted from

  • Lin, J.; Pillay, B.; Singh, S.
    Purification and biochemical characteristics of beta-D-glucosidase from a thermophilic fungus, Thermomyces lanuginosus-SSBP (1999), Biotechnol. Appl. Biochem., 30, 81-87 .
No PubMed abstract available
Search for more literature for 3.2.1.21

Activating Compound

Activating Compound Comment Organism Structure
cysteine
-
 Thermomyces lanuginosus

Inhibitors

Inhibitors Comment Organism Structure
cellobiose competitive Thermomyces lanuginosus
D-glucose competitive Thermomyces lanuginosus
Hg2+ 2 mM, complete inhibition Thermomyces lanuginosus
N-bromosuccinimide 2 mM, complete inhibition Thermomyces lanuginosus
Salicin competitive Thermomyces lanuginosus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.075
-
 4-nitrophenyl beta-D-glucopyranoside pH 6.5, 50°C Thermomyces lanuginosus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Thermomyces lanuginosus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
200000
-
 gel filtration Thermomyces lanuginosus

Organism

Organism UniProt Comment Textmining
Thermomyces lanuginosus
-
-
-
Thermomyces lanuginosus SSBP
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.6
-
 substrate cellobiose, pH 6.5, 50°C Thermomyces lanuginosus
9.65
-
 substrate 4-nitrophenyl beta-D-glucopyranoside, pH 6.5, 50°C Thermomyces lanuginosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Thermomyces lanuginosus 4-nitrophenol + D-glucose
-
 ?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Thermomyces lanuginosus SSBP 4-nitrophenol + D-glucose
-
 ?
cellobiose + H2O
-
 Thermomyces lanuginosus 2 D-glucose
-
 ?
cellobiose + H2O
-
 Thermomyces lanuginosus SSBP 2 D-glucose
-
 ?
additional information enzyme requires glucoside substrates containing a terminal, non-reducing beta-D-glucose residue. Enzyme exhibited little, but significant alpha-D-glucosidase activity and is capable of hydrolysing beta-1,6-linked diglucosides and gentiobiose Thermomyces lanuginosus ?
-
 ?
additional information enzyme requires glucoside substrates containing a terminal, non-reducing beta-D-glucose residue. Enzyme exhibited little, but significant alpha-D-glucosidase activity and is capable of hydrolysing beta-1,6-linked diglucosides and gentiobiose Thermomyces lanuginosus SSBP ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 105000, SDS-PAGE Thermomyces lanuginosus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
-
 Thermomyces lanuginosus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 30 min, stable Thermomyces lanuginosus
70
-
 30 min, complete loss of activity Thermomyces lanuginosus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
44.4
-
 4-nitrophenyl beta-D-glucopyranoside pH 6.5, 50°C Thermomyces lanuginosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Thermomyces lanuginosus

pH Stability

pH Stability pH Stability Maximum Comment Organism
5
-
 stable when the pH of the storage buffer is above 5.0 Thermomyces lanuginosus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.52
-
 cellobiose pH 6.5, 50°C Thermomyces lanuginosus
0.55
-
 D-glucose pH 6.5, 50°C Thermomyces lanuginosus
0.81
-
 Salicin pH 6.5, 50°C Thermomyces lanuginosus