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Literature summary for 3.2.1.21 extracted from

  • Takashima, S.; Nakamura, A.; Masaki, H.; Uozumi, T.
    Purification and characterization of cellulases from Humicola grisea (1996), Biosci. Biotechnol. Biochem., 60, 77-82 .
No PubMed abstract available
Search for more literature for 3.2.1.21

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.12
-
 4-nitrophenyl alpha-D-glucopyranoside isoform beta-glucosidase 6, pH 5.0, 50°C [Humicola] grisea var. thermoidea
0.16
-
 4-nitrophenyl alpha-D-glucopyranoside isoform beta-glucosidase 1, pH 5.0, 50°C [Humicola] grisea var. thermoidea
0.22
-
 4-nitrophenyl alpha-D-glucopyranoside isoform beta-glucosidase 3, pH 5.0, 50°C [Humicola] grisea var. thermoidea
0.25
-
 4-nitrophenyl alpha-D-glucopyranoside isoform beta-glucosidase 2, pH 5.0, 50°C [Humicola] grisea var. thermoidea
0.34
-
 4-nitrophenyl alpha-D-glucopyranoside isoform beta-glucosidase 4, pH 5.0, 50°C [Humicola] grisea var. thermoidea
0.56
-
 4-nitrophenyl alpha-D-glucopyranoside isoform beta-glucosidase 5, pH 5.0, 50°C [Humicola] grisea var. thermoidea

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
80000
-
 gel filtration, isoform beta-glucosidase 5 [Humicola] grisea var. thermoidea
300000
-
 gel filtration, isoform beta-glucosidase 1 [Humicola] grisea var. thermoidea
460000
-
 gel filtration, isoform beta-glucosidase 2 [Humicola] grisea var. thermoidea
460000
-
 gel filtration, isoform beta-glucosidase 3 [Humicola] grisea var. thermoidea

Organism

Organism UniProt Comment Textmining
[Humicola] grisea var. thermoidea
-
-
-
[Humicola] grisea var. thermoidea IFO 9854
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 [Humicola] grisea var. thermoidea

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.1
-
 isoform beta-glucosidase 4, pH 5.0, 50°C [Humicola] grisea var. thermoidea
11.9
-
 isoform beta-glucosidase 3, pH 5.0, 50°C [Humicola] grisea var. thermoidea
12
-
 isoform beta-glucosidase 2, pH 5.0, 50°C [Humicola] grisea var. thermoidea
17.5
-
 isoform beta-glucosidase 5, pH 5.0, 50°C [Humicola] grisea var. thermoidea
31.8
-
 isoform beta-glucosidase 6, pH 5.0, 50°C [Humicola] grisea var. thermoidea
76.7
-
 isoform beta-glucosidase 1, pH 5.0, 50°C [Humicola] grisea var. thermoidea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl alpha-D-glucopyranoside + H2O
-
 [Humicola] grisea var. thermoidea 4-nitrophenol + D-glucose
-
 ?
4-nitrophenyl alpha-D-glucopyranoside + H2O
-
 [Humicola] grisea var. thermoidea IFO 9854 4-nitrophenol + D-glucose
-
 ?
additional information beta-glucosidases show poor activity on 4-nitrophenyl beta-D-cellobioside or cellooligosaccharides [Humicola] grisea var. thermoidea ?
-
 ?
additional information beta-glucosidases show poor activity on 4-nitrophenyl beta-D-cellobioside or cellooligosaccharides [Humicola] grisea var. thermoidea IFO 9854 ?
-
 ?

Subunits

Subunits Comment Organism
oligomer x * 115000, isoform beta-glucosidase 1, x * 94000 plus x * 21000, isoform beta-glucosidase 2, x * 94000 plus x * 21000, isoform beta-glucosidase 3, x * 52000, isoform beta-glucosidase 4, x * 49000 plus x * 46000, isoform beta-glucosidase 5, x * 77000, isoform beta-glucosidase 6, SDS-PAGE [Humicola] grisea var. thermoidea