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Literature summary for 3.2.1.21 extracted from
- Purification and properties of the cellulases from the thermophilic fungus Thermoascus aurantiacus (1980), Biochem. J., 191, 83-94.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.52 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.0, 70°C | Thermoascus aurantiacus |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 87000 | - |
gel filtration | Thermoascus aurantiacus |
| 89000 | - |
1 * 89000, SDS-PAGE | Thermoascus aurantiacus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermoascus aurantiacus | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | contains 33% carbohydrate | Thermoascus aurantiacus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Thermoascus aurantiacus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, stable for at least a year | Thermoascus aurantiacus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Thermoascus aurantiacus | 4-nitrophenol + D-glucopyranose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 89000, SDS-PAGE | Thermoascus aurantiacus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
- |
Thermoascus aurantiacus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | 80 | 50°C: about 40% of maximal activity, 80°C: about 60% of maximal activity | Thermoascus aurantiacus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
1 h, stable | Thermoascus aurantiacus |
| 75 | - |
1 h, about 30% loss of activity | Thermoascus aurantiacus |
| 80 | - |
1 h, complete loss of activity | Thermoascus aurantiacus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Thermoascus aurantiacus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 6 | 4.0: about 50% of maximal activity, pH 6.0: about 35% of maximal activity | Thermoascus aurantiacus |
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Release 2023.1 (January 2023)
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