Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | complete inhibition at 1 mM | Humicola insolens | |
Cu2+ | 52% inhibition at 1 mM, 89% at 5 mM | Humicola insolens | |
D-glucose | competitive inhibition | Humicola insolens | |
D-mannose | competitive inhibition | Humicola insolens | |
D-xylose | competitive inhibition | Humicola insolens | |
Hg2+ | 33% inhibition at 1 mM, 48% at 5 mM | Humicola insolens | |
additional information | no inhibition by EDTA and insensitive to fructose, ribose, galactose, and L-arabinose up to 50 mM | Humicola insolens | |
Pb2+ | 45% inhibition at 1 mM, 61% at 5 mM | Humicola insolens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.081 | - |
4-nitrophenyl-beta-D-glucopyranoside | pH 5.0, 50°C | Humicola insolens | |
0.081 | - |
4-nitrophenyl-beta-D-xyloside | pH 5.0, 50°C | Humicola insolens | |
0.24 | - |
cellobiose | pH 5.0, 50°C | Humicola insolens | |
0.65 | - |
Salicin | pH 5.0, 50°C | Humicola insolens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no effect on enzyme activity by Ca2+, Sr2+, Co2+, Zn2+, Ni2+, Mg2+, and Mn2+ at up to 5 mM and by Fe2+ and Cr3+ at 1 mM, no metalloenzyme | Humicola insolens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
116000 | - |
4 * 116000, SDS-PAGE | Humicola insolens |
404000 | - |
gel filtration | Humicola insolens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cellobiose + H2O | Humicola insolens | - |
2 beta-D-glucose | - |
? | |
cellobiose + H2O | Humicola insolens RP86 | - |
2 beta-D-glucose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Humicola insolens | - |
from feces of Lama glama at the Ribeirao Preto Municipal Zoo (SP, Brazil) | - |
Humicola insolens RP86 | - |
from feces of Lama glama at the Ribeirao Preto Municipal Zoo (SP, Brazil) | - |
Purification (Comment) | Organism |
---|---|
native enzyme 23.6fold from crude culture extract by ammonium sulfate fractionation, gel filtration, anion exchange chromatography, ultrafiltration, again gel filtration, followed by hyrophobic interaction chromatography and ultrafiltration | Humicola insolens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | solid state fermentation | Humicola insolens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
45.6 | - |
purified enzyme, pH 5.0, 50°C, substrate cellobiose | Humicola insolens |
46.2 | - |
purified enzyme, pH 5.0, 50°C, substrate 4-nitrophenyl-beta-D-glucopyranoside | Humicola insolens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Humicola insolens | 4-nitrophenol + beta-D-glucose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Humicola insolens RP86 | 4-nitrophenol + beta-D-glucose | - |
? | |
4-nitrophenyl beta-D-xyloside + H2O | - |
Humicola insolens | 4-nitrophenol + D-xylose | - |
? | |
4-nitrophenyl beta-D-xyloside + H2O | - |
Humicola insolens RP86 | 4-nitrophenol + D-xylose | - |
? | |
cellobiose + H2O | - |
Humicola insolens | 2 beta-D-glucose | - |
? | |
cellobiose + H2O | - |
Humicola insolens RP86 | 2 beta-D-glucose | - |
? | |
additional information | no activity with 4-nitrophenyl-beta-D-galactopyranoside, 4-nitrophenyl-beta-D-furanoside, lactose, maltose, trehalose, and sucrose, and poor activity with carboxymethyl cellulose, and Avicel, substrate specificity, overview | Humicola insolens | ? | - |
? | |
additional information | no activity with 4-nitrophenyl-beta-D-galactopyranoside, 4-nitrophenyl-beta-D-furanoside, lactose, maltose, trehalose, and sucrose, and poor activity with carboxymethyl cellulose, and Avicel, substrate specificity, overview | Humicola insolens RP86 | ? | - |
? | |
salicin + H2O | - |
Humicola insolens | ? | - |
? | |
salicin + H2O | - |
Humicola insolens RP86 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 116000, SDS-PAGE | Humicola insolens |
Synonyms | Comment | Organism |
---|---|---|
BglHi2 | - |
Humicola insolens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
- |
Humicola insolens |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 75 | activity range, profile overview | Humicola insolens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
purified enzyme, pH 5.0, 60 min, stable at | Humicola insolens |
55 | - |
purified enzyme, pH 5.0, 60 min, 71% activity remaining | Humicola insolens |
60 | - |
purified enzyme, pH 5.0, half-life is 47.3 min | Humicola insolens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
10.2 | - |
4-nitrophenyl-beta-D-xyloside | pH 5.0, 50°C | Humicola insolens | |
343.5 | - |
4-nitrophenyl-beta-D-glucopyranoside | pH 5.0, 50°C | Humicola insolens | |
404.8 | - |
Salicin | pH 5.0, 50°C | Humicola insolens | |
3132 | - |
cellobiose | pH 5.0, 50°C | Humicola insolens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Humicola insolens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3.5 | 8 | activity range, 75% of maximal activity at pH 4.5 and pH 7.5, profile overview | Humicola insolens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
3.27 | - |
D-glucose | pH 5.0, 50°C | Humicola insolens | |
23.7 | - |
D-mannose | pH 5.0, 50°C | Humicola insolens | |
42.7 | - |
D-xylose | pH 5.0, 50°C | Humicola insolens |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Humicola insolens | isoelectric focusing | - |
5.6 |
General Information | Comment | Organism |
---|---|---|
additional information | synergism of the purified enzyme BglHi2 and Trichderma reesei cellulase in the hydrolysis reaction, overview | Humicola insolens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.16 | - |
4-nitrophenyl-beta-D-xyloside | pH 5.0, 50°C | Humicola insolens | |
622.7 | - |
Salicin | pH 5.0, 50°C | Humicola insolens | |
1305 | - |
cellobiose | pH 5.0, 50°C | Humicola insolens | |
4241 | - |
4-nitrophenyl-beta-D-glucopyranoside | pH 5.0, 50°C | Humicola insolens |