Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Weissella cibaria |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 5 mM Cu2+ reduces the activity to 46.1% | Weissella cibaria | |
E-64 | 1 mM E-64 reduces the activity to 78.3% | Weissella cibaria | |
Fe3+ | 5 mM Fe3+ reduces the activity to 55.8% | Weissella cibaria | |
Mn2+ | 5 mM Mn2+ reduces the activity to 86.9% | Weissella cibaria | |
additional information | not inhibited by EDTA and phenylmethylsulfonyl fluoride | Weissella cibaria | |
pepstatin | 1 mM pepstatin reduces the activity to 45.2% | Weissella cibaria | |
SDS | 1 mM SDS reduces the activity to 16.9% | Weissella cibaria | |
Zn2+ | 5 mM Cu2+ reduces the activity to 56.7% | Weissella cibaria |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.04 | - |
4-nitrophenyl beta-D-glucopyranoside | at pH 5.5 and 45°C | Weissella cibaria |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 5 mM Ca2+ slightly increases the activity to 108.2% | Weissella cibaria | |
additional information | not influenced by Co2+, K+, and Mg2+ | Weissella cibaria |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
49951 | - |
x * 49951, calculated from amino acid sequence | Weissella cibaria |
50000 | - |
x * 50000, SDS-PAGE | Weissella cibaria |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Weissella cibaria | I6TNE2 | - |
- |
Weissella cibaria 37 | I6TNE2 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni+2-nitrilotriacetic acid column chromatography | Weissella cibaria |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Weissella cibaria | 4-nitrophenol + D-glucopyranose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Weissella cibaria 37 | 4-nitrophenol + D-glucopyranose | - |
? | |
carboxymethyl cellulose + H2O | - |
Weissella cibaria | ? | - |
? | |
carboxymethyl cellulose + H2O | - |
Weissella cibaria 37 | ? | - |
? | |
cellobiose + H2O | - |
Weissella cibaria | ? | - |
? | |
cellobiose + H2O | - |
Weissella cibaria 37 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 50000, SDS-PAGE | Weissella cibaria |
? | x * 49951, calculated from amino acid sequence | Weissella cibaria |
Synonyms | Comment | Organism |
---|---|---|
beta-D-glucoside glucohydrolase | - |
Weissella cibaria |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Weissella cibaria |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 55 | more than 50% activity between 30 and 52°C. Activity decreases rapidly at temperature above 55°C | Weissella cibaria |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 50 | enzyme activity decreases rapidly during the first 10 min and then gradually decreases to about 30% until 60 min when incubated at 40-50°C | Weissella cibaria |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
30.63 | - |
4-nitrophenyl beta-D-glucopyranoside | at pH 5.5 and 45°C | Weissella cibaria |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
- |
Weissella cibaria |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 7.5 | more than 50% activity between pH 5.0 and 7.5 | Weissella cibaria |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Weissella cibaria | calculated from amino acid sequence | - |
5.1 |