Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ethanol | 10%, 1.1fold activation | Thermofilum pendens | |
methanol | 10%, 1.1fold activation | Thermofilum pendens |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Thermofilum pendens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
dimethyl sulfoxide | 30% 25% inhibition. 40% 47% inhibition. 50% 68% inhibition | Thermofilum pendens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.147 | - |
4-O-alpha-D-mannopyranosyl-D-mannopyranose | pH 3.5, 90°C | Thermofilum pendens | |
0.149 | - |
cellobiose | pH 3.5, 90°C | Thermofilum pendens | |
0.189 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 3.5, 90°C | Thermofilum pendens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 5 mM, 2fold activation | Thermofilum pendens | |
Cu2+ | 5 mM, 1.1fold activation | Thermofilum pendens | |
Mn2+ | 5 mM, 1.5fold activation | Thermofilum pendens | |
Zn2+ | 5 mM, 1.5fold activation | Thermofilum pendens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
77800 | - |
x * 77800, SDS-PAGE | Thermofilum pendens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermofilum pendens | A1S0B1 | - |
- |
Thermofilum pendens DSM 2475 | A1S0B1 | - |
- |
Purification (Comment) | Organism |
---|---|
Tpen_1494 is ligated into p6xHis119 downstream of the BLMA promoter to create p6xHisTpbgl. Recombinant Tpbgl, with eight additional amino acids (Met-Glu-(His)6) at the N-terminus, is expressed in Escherichia coli harboring p6xHisTpbgl | Thermofilum pendens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
7.6 | - |
crude extract of recombinant Escherichia coli, pH 3.5, 90°C | Thermofilum pendens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-D-glucopyranoside + H2O | when the enzyme iss incubated with 4-nitrophenyl-beta-D-glucopyranoside in the presence of ethanol and propanol, the glucose moiety is transferred to acceptor alcohols | Thermofilum pendens | 4-nitrophenol + beta-D-glucose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | when the enzyme iss incubated with 4-nitrophenyl-beta-D-glucopyranoside in the presence of ethanol and propanol, the glucose moiety is transferred to acceptor alcohols | Thermofilum pendens DSM 2475 | 4-nitrophenol + beta-D-glucose | - |
? | |
4-O-alpha-D-mannopyranosyl-D-mannopyranose + H2O | - |
Thermofilum pendens | 2 D-mannose | - |
? | |
4-O-alpha-D-mannopyranosyl-D-mannopyranose + H2O | - |
Thermofilum pendens DSM 2475 | 2 D-mannose | - |
? | |
cellobiose + H2O | - |
Thermofilum pendens | 2 beta-D-glucose | - |
? | |
cellobiose + H2O | - |
Thermofilum pendens DSM 2475 | 2 beta-D-glucose | - |
? | |
genistin + H2O | genistin solubilized in 10-40% dimethylsulfoxide is hydrolyzed to genistein with nearly 99% conversion, indicating that high concentrations of the water-insoluble isoflavone glycoside can be treated by the enzyme | Thermofilum pendens | beta-D-glucose + genistein | - |
? | |
genistin + H2O | genistin solubilized in 10-40% dimethylsulfoxide is hydrolyzed to genistein with nearly 99% conversion, indicating that high concentrations of the water-insoluble isoflavone glycoside can be treated by the enzyme | Thermofilum pendens DSM 2475 | beta-D-glucose + genistein | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 77800, SDS-PAGE | Thermofilum pendens |
Synonyms | Comment | Organism |
---|---|---|
Tpbgl | - |
Thermofilum pendens |
Tpen_1494 | locus name | Thermofilum pendens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
- |
Thermofilum pendens |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | 100 | 60°C: about 70% of maximal activity, 100°C: about 75% of maxim al activity | Thermofilum pendens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
most heat stable under extremely acidic conditions, pH 3.5 | Thermofilum pendens |
85 | 90 | the enzyme retains nearly 100% of its original activity over 120 min of incubation | Thermofilum pendens |
90 | - |
pH 3.5-4.5, 1 or 2 h, more than 80% of the maximum activity is retained | Thermofilum pendens |
95 | - |
50% activity remains after incubation for 60 min | Thermofilum pendens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.038 | - |
4-O-alpha-D-mannopyranosyl-D-mannopyranose | pH 3.5, 90°C | Thermofilum pendens | |
0.317 | - |
cellobiose | pH 3.5, 90°C | Thermofilum pendens | |
0.577 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 3.5, 90°C | Thermofilum pendens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3.5 | - |
incubation at 90°C | Thermofilum pendens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 5 | pH 3.0: about 50% of maximal activity, pH 5.0: about 80% of maximal activity | Thermofilum pendens |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3.5 | 4 | 90°C, 1 or 2 h, more than 80% of the maximum activity is retained | Thermofilum pendens |
4.5 | 6 | 90°C, 1 or 2 h, 60% of the activity is retained | Thermofilum pendens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.26 | - |
4-O-alpha-D-mannopyranosyl-D-mannopyranose | pH 3.5, 90°C | Thermofilum pendens | |
2.13 | - |
cellobiose | pH 3.5, 90°C | Thermofilum pendens | |
3.05 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 3.5, 90°C | Thermofilum pendens |