Application | Comment | Organism |
---|---|---|
synthesis | preparation of lactose-free pasteurized milk with a recombinant thermostable beta-glucosidase | Pyrococcus furiosus |
Cloned (Comment) | Organism |
---|---|
cloned in frame with the Saccharomyces cerecisiae a-factor secretory signal and expressed in Pichia pastoris strain X-33 | Pyrococcus furiosus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 1 mM, 22% inhibition, not significantly stimulated or inhibited by other divalent cations | Pyrococcus furiosus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
120000 | - |
dimer | Pyrococcus furiosus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus furiosus | Q51723 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Pyrococcus furiosus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
694.6 | - |
pH and temperature not specified in the publication | Pyrococcus furiosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
lactose + H2O | preparation of lactose-free pasteurized milk with a recombinant thermostable beta-glucosidase | Pyrococcus furiosus | D-glucose + D-galactose | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
100 | - |
- |
Pyrococcus furiosus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | 110 | 70°C: about 45% of maximal activity, 110°C: about 30% of maximal activity | Pyrococcus furiosus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 120 | the enzyme retains more than 80% of its maximum activity from 30°C to 120°C | Pyrococcus furiosus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Pyrococcus furiosus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 7 | pH 5.0: about 60% of maximal activity, pH 7.0: about 80% of maximal activity | Pyrococcus furiosus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 8 | 37°C, 1 h incubation, the enzyme retains more than 80% of its maximum activity | Pyrococcus furiosus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Pyrococcus furiosus | - |
- |
4.4 |