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Literature summary for 3.2.1.21 extracted from

  • Fan, T.W.; Conn, E.E.
    Isolation and characterization of two cyanogenic beta-glucosidases from flax seeds (1985), Arch. Biochem. Biophys., 243, 361-73.
    View publication on PubMed
Search for more literature for 3.2.1.21

Inhibitors

Inhibitors Comment Organism Structure
D-glucono-1,5-lactone i.e. delta-gluconolactone Linum usitatissimum
linustatin
-
 Linum usitatissimum
neolinustatin
-
 Linum usitatissimum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6
-
 linustatin pH 6, 30°C Linum usitatissimum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
58000
-
 alpha=39000 Da, beta=19000 Da, chromatography on P-100 and S-300 columns Linum usitatissimum

Organism

Organism UniProt Comment Textmining
Linum usitatissimum
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Linum usitatissimum

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
linustatin + H2O
-
 Linum usitatissimum linamarin + D-glucose
-
 ?
neolinustatin + H2O
-
 Linum usitatissimum lotaustralin + D-glucose
-
 ?

Subunits

Subunits Comment Organism
heterodimer alphabeta, 1*39000, 1*19000 Linum usitatissimum

Synonyms

Synonyms Comment Organism
linustatinase
-
 Linum usitatissimum

pH Range

pH Minimum pH Maximum Comment Organism
4 6
-
 Linum usitatissimum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.11
-
 D-glucono-1,5-lactone pH 6, 30°C Linum usitatissimum
5
-
 neolinustatin pH 6, 30°C Linum usitatissimum
10
-
 linustatin pH 6, 30°C Linum usitatissimum