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Literature summary for 3.2.1.21 extracted from

  • Hong, M.R.; Kim, Y.S.; Park, C.S.; Lee, J.K.; Kim, Y.S.; Oh, D.K.
    Characterization of a recombinant beta-glucosidase from the thermophilic bacterium Caldicellulosiruptor saccharolyticus (2009), J. Biosci. Bioeng., 108, 36-40.
    View publication on PubMed
Search for more literature for 3.2.1.21

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.44
-
 4-nitrophenyl-beta-D-fucopyranoside in 50 mM citrate buffer, at pH 5.5 and 70°C Caldicellulosiruptor saccharolyticus
0.67
-
 4-nitrophenyl-beta-D-glucopyranoside in 50 mM citrate buffer, at pH 5.5 and 70°C Caldicellulosiruptor saccharolyticus
1.93
-
 4-nitrophenyl-beta-D-galactopyranoside in 50 mM citrate buffer, at pH 5.5 and 70°C Caldicellulosiruptor saccharolyticus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54000
-
 2 * 54000, SDS-PAGE Caldicellulosiruptor saccharolyticus
108000
-
 gel filtration Caldicellulosiruptor saccharolyticus

Organism

Organism UniProt Comment Textmining
Caldicellulosiruptor saccharolyticus A4XIG7 strain DSM 8903
-

Purification (Commentary)

Purification (Comment) Organism
heat treatment and His-Trap affinity chromatography Caldicellulosiruptor saccharolyticus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.08
-
 crude extract, at 70°C Caldicellulosiruptor saccharolyticus
0.28
-
 after 163fold purification, using 2-nitrophenyl-beta-D-galactopyranoside as substrate, at 70°C Caldicellulosiruptor saccharolyticus
0.3
-
 after 163fold purification, using 2-nitrophenyl-beta-D-glucopyranoside as substrate, at 70°C Caldicellulosiruptor saccharolyticus
0.32
-
 after 163fold purification, using 4-nitrophenyl-beta-D-xylopyranoside as substrate, at 70°C Caldicellulosiruptor saccharolyticus
0.75
-
 after 163fold purification, using 4-nitrophenyl-beta-D-lactopyranoside as substrate, at 70°C Caldicellulosiruptor saccharolyticus
1.3
-
 after 163fold purification, using 4-nitrophenyl-beta-D-cellobioside as substrate, at 70°C Caldicellulosiruptor saccharolyticus
6.1
-
 after 163fold purification, using 4-nitrophenyl-beta-D-galactopyranoside as substrate, at 70°C Caldicellulosiruptor saccharolyticus
6.8
-
 after 163fold purification, using cellopentaose as substrate, at 70°C Caldicellulosiruptor saccharolyticus
9.5
-
 after 163fold purification, using cellotriose as substrate, at 70°C Caldicellulosiruptor saccharolyticus
10.8
-
 after 163fold purification, using cellotetraose as substrate, at 70°C Caldicellulosiruptor saccharolyticus
13
-
 after 163fold purification, using 4-nitrophenyl-beta-D-glucopyranoside as substrate, at 70°C Caldicellulosiruptor saccharolyticus
14.7
-
 after 163fold purification, using 4-nitrophenyl-beta-D-fucopyranoside as substrate, at 70°C Caldicellulosiruptor saccharolyticus
16.2
-
 after 163fold purification, using cellobiose as substrate, at 70°C Caldicellulosiruptor saccharolyticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-nitrophenyl beta-D-galactopyranoside + H2O
-
 Caldicellulosiruptor saccharolyticus 2-nitrophenol + beta-D-galactose
-
 ?
2-nitrophenyl beta-D-glucopyranoside + H2O
-
 Caldicellulosiruptor saccharolyticus 2-nitrophenol + D-glucopyranose
-
 ?
4-nitrophenyl beta-D-cellobioside + H2O
-
 Caldicellulosiruptor saccharolyticus 4-nitrophenol + cellobiose
-
 ?
4-nitrophenyl beta-D-fucopyranoside + H2O
-
 Caldicellulosiruptor saccharolyticus 4-nitrophenol + beta-D-fucose
-
 ?
4-nitrophenyl beta-D-galactopyranoside + H2O
-
 Caldicellulosiruptor saccharolyticus 4-nitrophenol + beta-D-galactose
-
 ?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Caldicellulosiruptor saccharolyticus 4-nitrophenol + D-glucopyranose
-
 ?
4-nitrophenyl beta-D-lactopyranoside + H2O
-
 Caldicellulosiruptor saccharolyticus 4-nitrophenol + beta-D-lactose
-
 ?
4-nitrophenyl beta-D-xylopyranoside + H2O
-
 Caldicellulosiruptor saccharolyticus 4-nitrophenol + beta-D-xylose
-
 ?
cellobiose + H2O
-
 Caldicellulosiruptor saccharolyticus 2 beta-D-glucose
-
 ?
cellobiose + H2O highest specific activity Caldicellulosiruptor saccharolyticus ?
-
 ?
cellopentaose + H2O
-
 Caldicellulosiruptor saccharolyticus ?
-
 ?
cellotetraose + H2O
-
 Caldicellulosiruptor saccharolyticus ?
-
 ?
cellotriose + H2O
-
 Caldicellulosiruptor saccharolyticus ?
-
 ?
additional information cello-oligosaccharides from n equal 2 to 5 as substrates using 4 mM each sugar and 3 units/mg of enzyme are completely hydrolyzed to beta-D-glucose at 70°C within 16 h Caldicellulosiruptor saccharolyticus ?
-
 ?
additional information the enzyme does not hydrolyze 4-nitrophenyl-alpha-D-glucopyranoside, 4-nitrophenyl-alpha-D-galactopyranoside, 4-nitrophenyl-alpha-L-arabinopyranoside, 4-nitrophenyl-beta-D-maltopyranoside, 4-nitrophenyl-beta-D-mannopyranoside, and 4-nitrophenyl-beta-L-arabinopyranoside Caldicellulosiruptor saccharolyticus ?
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 54000, SDS-PAGE Caldicellulosiruptor saccharolyticus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
 Caldicellulosiruptor saccharolyticus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60 80 the half-lives of the enzyme at 60, 70, and 80°C are 250, 24.3, and 0.4 h Caldicellulosiruptor saccharolyticus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
82
-
 4-nitrophenyl-beta-D-galactopyranoside in 50 mM citrate buffer, at pH 5.5 and 70°C Caldicellulosiruptor saccharolyticus
387
-
 4-nitrophenyl-beta-D-glucopyranoside in 50 mM citrate buffer, at pH 5.5 and 70°C Caldicellulosiruptor saccharolyticus
516
-
 4-nitrophenyl-beta-D-fucopyranoside in 50 mM citrate buffer, at pH 5.5 and 70°C Caldicellulosiruptor saccharolyticus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 Caldicellulosiruptor saccharolyticus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
42
-
 4-nitrophenyl-beta-D-galactopyranoside in 50 mM citrate buffer, at pH 5.5 and 70°C Caldicellulosiruptor saccharolyticus
579
-
 4-nitrophenyl-beta-D-glucopyranoside in 50 mM citrate buffer, at pH 5.5 and 70°C Caldicellulosiruptor saccharolyticus
1185
-
 4-nitrophenyl-beta-D-fucopyranoside in 50 mM citrate buffer, at pH 5.5 and 70°C Caldicellulosiruptor saccharolyticus