Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.21 extracted from

  • Tomassi, M.; Rozenfeld, J.; Gonçalves, L.; Marana, S.
    Characterization of the interdependency between residues that bind the substrate in a beta-glycosidase (2010), Braz. J. Med. Biol. Res., 43, 8-12.
    View publication on PubMed
Search for more literature for 3.2.1.21

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Spodoptera frugiperda

Protein Variants

Protein Variants Comment Organism
E451A the double mutant shows decreased activity compared to the wild type enzyme Spodoptera frugiperda
E451S the double mutant shows decreased activity compared to the wild type enzyme Spodoptera frugiperda
Q39E the double mutant shows decreased activity compared to the wild type enzyme Spodoptera frugiperda
Q39E/E451A the double mutant shows decreased activity compared to the wild type enzyme Spodoptera frugiperda
Q39E/E451S the double mutant shows decreased activity compared to the wild type enzyme Spodoptera frugiperda
Q39N the double mutant shows decreased activity compared to the wild type enzyme Spodoptera frugiperda
Q39N/E451S the double mutant shows decreased activity compared to the wild type enzyme Spodoptera frugiperda

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.11
-
 4-nitrophenyl-beta-D-galactoside mutant enzyme Q39N/E451S, at 30°C Spodoptera frugiperda
0.22
-
 4-nitrophenyl-beta-D-galactoside mutant enzyme Q39E/E451A, at 30°C Spodoptera frugiperda
0.26
-
 4-nitrophenyl-beta-D-fucoside mutant enzyme Q39N, at 30°C Spodoptera frugiperda
0.38
-
 4-nitrophenyl-beta-D-galactoside mutant enzyme Q39E/E451S, at 30°C Spodoptera frugiperda
0.49
-
 4-nitrophenyl-beta-D-fucoside wild type enzyme, at 30°C Spodoptera frugiperda
0.5
-
 4-nitrophenyl-beta-D-galactoside mutant enzyme E451A, at 30°C Spodoptera frugiperda
0.68
-
 4-nitrophenyl-beta-D-fucoside mutant enzyme E451S, at 30°C Spodoptera frugiperda
1.5
-
 4-nitrophenyl-beta-D-galactoside mutant enzyme Q39N, at 30°C Spodoptera frugiperda
1.53
-
 4-nitrophenyl-beta-D-fucoside mutant enzyme E451A, at 30°C Spodoptera frugiperda
1.7
-
 4-nitrophenyl-beta-D-galactoside mutant enzyme E451S, at 30°C Spodoptera frugiperda
2
-
 4-nitrophenyl-beta-D-galactoside wild type enzyme, at 30°C Spodoptera frugiperda
2.2
-
 4-nitrophenyl-beta-D-fucoside mutant enzyme Q39N/E451S, at 30°C Spodoptera frugiperda
2.3
-
 4-nitrophenyl-beta-D-fucoside mutant enzyme Q39E, at 30°C Spodoptera frugiperda
2.3
-
 4-nitrophenyl-beta-D-fucoside mutant enzyme Q39E/E451S, at 30°C Spodoptera frugiperda
2.7
-
 4-nitrophenyl-beta-D-fucoside mutant enzyme Q39E/E451A, at 30°C Spodoptera frugiperda
2.8
-
 4-nitrophenyl-beta-D-galactoside mutant enzyme Q39E, at 30°C Spodoptera frugiperda

Organism

Organism UniProt Comment Textmining
Spodoptera frugiperda
-
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap column chromatography Spodoptera frugiperda

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-D-fucoside + H2O
-
 Spodoptera frugiperda 4-nitrophenol + beta-D-fucose
-
 ?
4-nitrophenyl beta-D-galactoside + H2O
-
 Spodoptera frugiperda 4-nitrophenol + beta-D-galactose
-
 ?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Spodoptera frugiperda 4-nitrophenol + D-glucopyranose
-
 ?

Synonyms

Synonyms Comment Organism
betagly
-
 Spodoptera frugiperda

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.000285
-
 4-nitrophenyl-beta-D-galactoside mutant enzyme E451A, at 30°C Spodoptera frugiperda
0.00038
-
 4-nitrophenyl-beta-D-galactoside mutant enzyme Q39N, at 30°C Spodoptera frugiperda
0.0004
-
 4-nitrophenyl-beta-D-fucoside mutant enzyme Q39N/E451S, at 30°C Spodoptera frugiperda
0.00053
-
 4-nitrophenyl-beta-D-fucoside mutant enzyme E451S, at 30°C Spodoptera frugiperda
0.0006
-
 4-nitrophenyl-beta-D-galactoside mutant enzyme Q39N/E451S, at 30°C Spodoptera frugiperda
0.00061
-
 4-nitrophenyl-beta-D-fucoside mutant enzyme E451A, at 30°C Spodoptera frugiperda
0.00093
-
 4-nitrophenyl-beta-D-galactoside mutant enzyme E451S, at 30°C Spodoptera frugiperda
0.0036
-
 4-nitrophenyl-beta-D-galactoside mutant enzyme Q39E/E451A, at 30°C Spodoptera frugiperda
0.0065
-
 4-nitrophenyl-beta-D-galactoside mutant enzyme Q39E/E451S, at 30°C Spodoptera frugiperda
0.022
-
 4-nitrophenyl-beta-D-fucoside mutant enzyme Q39N, at 30°C Spodoptera frugiperda
0.0236
-
 4-nitrophenyl-beta-D-fucoside mutant enzyme Q39E/E451A, at 30°C Spodoptera frugiperda
0.0342
-
 4-nitrophenyl-beta-D-fucoside mutant enzyme Q39E/E451S, at 30°C Spodoptera frugiperda
0.0377
-
 4-nitrophenyl-beta-D-galactoside mutant enzyme Q39E, at 30°C Spodoptera frugiperda
0.35
-
 4-nitrophenyl-beta-D-galactoside wild type enzyme, at 30°C Spodoptera frugiperda
1.49
-
 4-nitrophenyl-beta-D-fucoside mutant enzyme Q39E, at 30°C Spodoptera frugiperda
3.3
-
 4-nitrophenyl-beta-D-fucoside wild type enzyme, at 30°C Spodoptera frugiperda

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.00018
-
 4-nitrophenyl-beta-D-fucoside mutant enzyme Q39N/E451S, at 30°C Spodoptera frugiperda
0.00025
-
 4-nitrophenyl-beta-D-galactoside mutant enzyme Q39N, at 30°C Spodoptera frugiperda
0.00053
-
 4-nitrophenyl-beta-D-galactoside mutant enzyme E451S, at 30°C Spodoptera frugiperda
0.00057
-
 4-nitrophenyl-beta-D-galactoside mutant enzyme E451A, at 30°C Spodoptera frugiperda
0.0039
-
 4-nitrophenyl-beta-D-fucoside mutant enzyme E451A, at 30°C Spodoptera frugiperda
0.005
-
 4-nitrophenyl-beta-D-galactoside mutant enzyme Q39N/E451S, at 30°C Spodoptera frugiperda
0.0087
-
 4-nitrophenyl-beta-D-fucoside mutant enzyme Q39E/E451A, at 30°C Spodoptera frugiperda
0.0134
-
 4-nitrophenyl-beta-D-galactoside mutant enzyme Q39E, at 30°C Spodoptera frugiperda
0.014
-
 4-nitrophenyl-beta-D-fucoside mutant enzyme Q39E/E451S, at 30°C Spodoptera frugiperda
0.016
-
 4-nitrophenyl-beta-D-galactoside mutant enzyme Q39E/E451A, at 30°C Spodoptera frugiperda
0.017
-
 4-nitrophenyl-beta-D-galactoside mutant enzyme Q39E/E451S, at 30°C Spodoptera frugiperda
0.027
-
 4-nitrophenyl-beta-D-fucoside mutant enzyme E451S, at 30°C Spodoptera frugiperda
0.084
-
 4-nitrophenyl-beta-D-fucoside mutant enzyme Q39N, at 30°C Spodoptera frugiperda
0.17
-
 4-nitrophenyl-beta-D-galactoside wild type enzyme, at 30°C Spodoptera frugiperda
0.64
-
 4-nitrophenyl-beta-D-fucoside mutant enzyme Q39E, at 30°C Spodoptera frugiperda
6.7
-
 4-nitrophenyl-beta-D-fucoside wild type enzyme, at 30°C Spodoptera frugiperda