Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.21 extracted from

  • Hayase, M.; Maekawa, A.; Yubisui, T.; Minami, Y.
    Properties, intracellular localization, and stage-specific expression of membrane-bound beta-glucosidase, BglM1, from Physarum polycephalum (2008), Int. J. Biochem. Cell Biol., 40, 2141-2150.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
castanospermine
-
Physarum polycephalum
D-glucono-1,5-lactone
-
Physarum polycephalum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
4-nitrophenyl beta-D-glucopyranoside 130000 Da form, pH 4.5, 37°C Physarum polycephalum
0.1
-
2-nitrophenyl beta-D-glucopyranoside 130000 Da form, pH 4.5, 37°C Physarum polycephalum
0.11
-
2-nitrophenyl beta-D-glucopyranoside 230000 Da form, pH 4.5, 37°C Physarum polycephalum
0.19
-
4-nitrophenyl beta-D-glucopyranoside 230000 Da form, pH 4.5, 37°C Physarum polycephalum
0.2
-
cellohexaose 230000 Da form, pH 4.5, 37°C Physarum polycephalum
0.26
-
cellohexaose 130000 Da form, pH 4.5, 37°C Physarum polycephalum
0.43
-
gentiobiose 130000 Da form, pH 4.5, 37°C Physarum polycephalum
0.52
-
gentiobiose 230000 Da form, pH 4.5, 37°C Physarum polycephalum
5.26
-
cellobiose 230000 Da form, pH 4.5, 37°C Physarum polycephalum
9.41
-
cellobiose 130000 Da form, pH 4.5, 37°C Physarum polycephalum

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Physarum polycephalum 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
130000
-
x * 130000, calculated, x * 130000, SDS-PAGE, and x * 230000, SDS-PAGE of enzyme purified in presence of Ca2+ or enzyme in crude extract Physarum polycephalum
230000
-
x * 130000, calculated, x * 130000, SDS-PAGE, and x * 230000, SDS-PAGE of enzyme purified in presence of Ca2+ or enzyme in crude extract Physarum polycephalum

Organism

Organism UniProt Comment Textmining
Physarum polycephalum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Physarum polycephalum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-nitrophenyl beta-D-glucopyranoside + H2O
-
Physarum polycephalum 2-nitrophenol + beta-D-glucose
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
Physarum polycephalum 4-nitrophenol + beta-D-glucose
-
?
cellobiose + H2O
-
Physarum polycephalum 2 beta-D-glucose
-
?
cellohexaose + 5 H2O
-
Physarum polycephalum 6 D-glucose
-
?
gentiobiose + H2O
-
Physarum polycephalum 2 beta-D-glucose
-
?

Subunits

Subunits Comment Organism
More enzyme exists in a 130000 Da and in a 230000 Da form. Kinetic data for some substrates are different for the two forms, while no difference is observed in optimum pH and temperature Physarum polycephalum
oligomer x * 130000, calculated, x * 130000, SDS-PAGE, and x * 230000, SDS-PAGE of enzyme purified in presence of Ca2+ or enzyme in crude extract Physarum polycephalum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
both 13000 Da and 230000 Da form Physarum polycephalum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
30% residual activity, both 13000 Da and 230000 Da form Physarum polycephalum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1
-
2-nitrophenyl beta-D-glucopyranoside 230000 Da form, pH 4.5, 37°C Physarum polycephalum
5
-
cellohexaose 230000 Da form, pH 4.5, 37°C Physarum polycephalum
8
-
cellohexaose 130000 Da form, pH 4.5, 37°C Physarum polycephalum
13
-
2-nitrophenyl beta-D-glucopyranoside 130000 Da form, pH 4.5, 37°C Physarum polycephalum
13
-
4-nitrophenyl beta-D-glucopyranoside 230000 Da form, pH 4.5, 37°C Physarum polycephalum
17
-
gentiobiose 230000 Da form, pH 4.5, 37°C Physarum polycephalum
20
-
gentiobiose 130000 Da form, pH 4.5, 37°C Physarum polycephalum
37
-
cellobiose 230000 Da form, pH 4.5, 37°C Physarum polycephalum
103
-
cellobiose 130000 Da form, pH 4.5, 37°C Physarum polycephalum
110
-
4-nitrophenyl beta-D-glucopyranoside 130000 Da form, pH 4.5, 37°C Physarum polycephalum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
both 13000 Da and 230000 Da form Physarum polycephalum

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.03
-
pH 4.5, 37°C, both 13000 Da and 230000 Da form Physarum polycephalum castanospermine
0.16
-
pH 4.5, 37°C, both 13000 Da and 230000 Da form Physarum polycephalum D-glucono-1,5-lactone