Inhibitors | Comment | Organism | Structure |
---|---|---|---|
castanospermine | - |
Physarum polycephalum | |
D-glucono-1,5-lactone | - |
Physarum polycephalum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
4-nitrophenyl beta-D-glucopyranoside | 130000 Da form, pH 4.5, 37°C | Physarum polycephalum | |
0.1 | - |
2-nitrophenyl beta-D-glucopyranoside | 130000 Da form, pH 4.5, 37°C | Physarum polycephalum | |
0.11 | - |
2-nitrophenyl beta-D-glucopyranoside | 230000 Da form, pH 4.5, 37°C | Physarum polycephalum | |
0.19 | - |
4-nitrophenyl beta-D-glucopyranoside | 230000 Da form, pH 4.5, 37°C | Physarum polycephalum | |
0.2 | - |
cellohexaose | 230000 Da form, pH 4.5, 37°C | Physarum polycephalum | |
0.26 | - |
cellohexaose | 130000 Da form, pH 4.5, 37°C | Physarum polycephalum | |
0.43 | - |
gentiobiose | 130000 Da form, pH 4.5, 37°C | Physarum polycephalum | |
0.52 | - |
gentiobiose | 230000 Da form, pH 4.5, 37°C | Physarum polycephalum | |
5.26 | - |
cellobiose | 230000 Da form, pH 4.5, 37°C | Physarum polycephalum | |
9.41 | - |
cellobiose | 130000 Da form, pH 4.5, 37°C | Physarum polycephalum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Physarum polycephalum | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
130000 | - |
x * 130000, calculated, x * 130000, SDS-PAGE, and x * 230000, SDS-PAGE of enzyme purified in presence of Ca2+ or enzyme in crude extract | Physarum polycephalum |
230000 | - |
x * 130000, calculated, x * 130000, SDS-PAGE, and x * 230000, SDS-PAGE of enzyme purified in presence of Ca2+ or enzyme in crude extract | Physarum polycephalum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Physarum polycephalum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Physarum polycephalum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-nitrophenyl beta-D-glucopyranoside + H2O | - |
Physarum polycephalum | 2-nitrophenol + beta-D-glucose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Physarum polycephalum | 4-nitrophenol + beta-D-glucose | - |
? | |
cellobiose + H2O | - |
Physarum polycephalum | 2 beta-D-glucose | - |
? | |
cellohexaose + 5 H2O | - |
Physarum polycephalum | 6 D-glucose | - |
? | |
gentiobiose + H2O | - |
Physarum polycephalum | 2 beta-D-glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | enzyme exists in a 130000 Da and in a 230000 Da form. Kinetic data for some substrates are different for the two forms, while no difference is observed in optimum pH and temperature | Physarum polycephalum |
oligomer | x * 130000, calculated, x * 130000, SDS-PAGE, and x * 230000, SDS-PAGE of enzyme purified in presence of Ca2+ or enzyme in crude extract | Physarum polycephalum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
both 13000 Da and 230000 Da form | Physarum polycephalum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
30% residual activity, both 13000 Da and 230000 Da form | Physarum polycephalum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1 | - |
2-nitrophenyl beta-D-glucopyranoside | 230000 Da form, pH 4.5, 37°C | Physarum polycephalum | |
5 | - |
cellohexaose | 230000 Da form, pH 4.5, 37°C | Physarum polycephalum | |
8 | - |
cellohexaose | 130000 Da form, pH 4.5, 37°C | Physarum polycephalum | |
13 | - |
2-nitrophenyl beta-D-glucopyranoside | 130000 Da form, pH 4.5, 37°C | Physarum polycephalum | |
13 | - |
4-nitrophenyl beta-D-glucopyranoside | 230000 Da form, pH 4.5, 37°C | Physarum polycephalum | |
17 | - |
gentiobiose | 230000 Da form, pH 4.5, 37°C | Physarum polycephalum | |
20 | - |
gentiobiose | 130000 Da form, pH 4.5, 37°C | Physarum polycephalum | |
37 | - |
cellobiose | 230000 Da form, pH 4.5, 37°C | Physarum polycephalum | |
103 | - |
cellobiose | 130000 Da form, pH 4.5, 37°C | Physarum polycephalum | |
110 | - |
4-nitrophenyl beta-D-glucopyranoside | 130000 Da form, pH 4.5, 37°C | Physarum polycephalum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
both 13000 Da and 230000 Da form | Physarum polycephalum |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.03 | - |
pH 4.5, 37°C, both 13000 Da and 230000 Da form | Physarum polycephalum | castanospermine | |
0.16 | - |
pH 4.5, 37°C, both 13000 Da and 230000 Da form | Physarum polycephalum | D-glucono-1,5-lactone |