Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.21 extracted from

  • Mendonca, L.M.; Marana, S.R.
    The role in the substrate specificity and catalysis of residues forming the substrate aglycone-binding site of a beta-glycosidase (2008), FEBS J., 275, 2536-2547.
    View publication on PubMed
Search for more literature for 3.2.1.21

Cloned(Commentary)

Cloned (Comment) Organism
-
 Spodoptera frugiperda

Protein Variants

Protein Variants Comment Organism
E190Q mutant construcuted for analysis of the relative contribution of the residue to specificity and catalysis. E190 favors the binding of the initial portion of alkyl-type aglycones Spodoptera frugiperda
E194A mutant construcuted for analysis of the relative contribution of the residue to specificity and catalysis. E190 favors the binding of the initial portion of alkyl-type aglycones Spodoptera frugiperda
K201A mutant construcuted for analysis of the relative contribution of the residue to specificity and catalysis. K201 is relevant for binding of D-glucose units Spodoptera frugiperda
K201F mutant construcuted for analysis of the relative contribution of the residue to specificity and catalysis. K201 is relevant for binding of D-glucose units Spodoptera frugiperda
M453A mutant construcuted for analysis of the relative contribution of the residue to specificity and catalysis. M453 favors the binding of the second D-glucose unit of oligosaccharidic aglycones Spodoptera frugiperda

Inhibitors

Inhibitors Comment Organism Structure
cellobiose
-
 Spodoptera frugiperda
cellopentaose
-
 Spodoptera frugiperda
cellotetraose
-
 Spodoptera frugiperda
cellotriose
-
 Spodoptera frugiperda
heptyl beta-D-glucoside
-
 Spodoptera frugiperda
hexyl beta-D-glucoside
-
 Spodoptera frugiperda
methyl beta-D-glucoside
-
 Spodoptera frugiperda
nonyl beta-D-glucoside
-
 Spodoptera frugiperda
octyl beta-D-glucoside
-
 Spodoptera frugiperda
phenyl beta-D-glucoside
-
 Spodoptera frugiperda

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.08
-
 4-methylumbeliferyl beta-D-glucoside mutant K201F, 30°C, pH 6.0 Spodoptera frugiperda
0.38
-
 4-nitrophenyl beta-D-fucoside mutant E194A, 30°C, pH 6.0 Spodoptera frugiperda
0.59
-
 4-nitrophenyl beta-D-fucoside mutant E190A, 30°C, pH 6.0 Spodoptera frugiperda
0.62
-
 4-nitrophenyl beta-D-fucoside mutant K201F, 30°C, pH 6.0 Spodoptera frugiperda
0.65
-
 4-nitrophenyl beta-D-fucoside mutant M453A, 30°C, pH 6.0 Spodoptera frugiperda
0.66
-
 4-nitrophenyl beta-D-fucoside mutant K201F, 30°C, pH 6.0 Spodoptera frugiperda
0.67
-
 4-nitrophenyl beta-D-fucoside wild-type, 30°C, pH 6.0 Spodoptera frugiperda
0.71
-
 4-nitrophenyl beta-D-fucoside mutant K201A, 30°C, pH 6.0 Spodoptera frugiperda
0.9
-
 4-nitrophenyl beta-D-glucoside wild-type, 30°C, pH 6.0 Spodoptera frugiperda
1.04
-
 4-nitrophenyl beta-D-xyloside mutant K201F, 30°C, pH 6.0 Spodoptera frugiperda
1.05
-
 4-nitrophenyl beta-D-glucoside mutant E190A, 30°C, pH 6.0 Spodoptera frugiperda
1.2
-
 4-nitrophenyl beta-D-glucoside mutant E194A, 30°C, pH 6.0 Spodoptera frugiperda
1.2
-
 4-nitrophenyl beta-D-glucoside mutant K201F, 30°C, pH 6.0 Spodoptera frugiperda
1.26
-
 4-nitrophenyl beta-D-glucoside mutant M453A, 30°C, pH 6.0 Spodoptera frugiperda
1.56
-
 4-nitrophenyl beta-D-xyloside wild-type, 30°C, pH 6.0 Spodoptera frugiperda
1.7
-
 4-methylumbeliferyl beta-D-glucoside mutant M453A, 30°C, pH 6.0 Spodoptera frugiperda
2.1
-
 4-nitrophenyl beta-D-glucoside mutant E190Q, 30°C, pH 6.0 Spodoptera frugiperda
2.3
-
 4-methylumbeliferyl beta-D-glucoside wild-type, 30°C, pH 6.0 Spodoptera frugiperda
2.4
-
 4-nitrophenyl beta-D-xyloside mutant K201A, 30°C, pH 6.0 Spodoptera frugiperda
2.6
-
 4-methylumbeliferyl beta-D-glucoside mutant E194A, 30°C, pH 6.0 Spodoptera frugiperda
2.6
-
 4-nitrophenyl beta-D-glucoside mutant K201A, 30°C, pH 6.0 Spodoptera frugiperda
2.6
-
 4-methylumbeliferyl beta-D-glucoside mutant K201A, 30°C, pH 6.0 Spodoptera frugiperda
2.9
-
 4-nitrophenyl beta-D-galactoside wild-type, 30°C, pH 6.0 Spodoptera frugiperda
3.6
-
 4-nitrophenyl beta-D-xyloside mutant M453A, 30°C, pH 6.0 Spodoptera frugiperda
4.4
-
 4-nitrophenyl beta-D-galactoside mutant M453A, 30°C, pH 6.0 Spodoptera frugiperda
4.7
-
 4-nitrophenyl beta-D-xyloside mutant E190Q, 30°C, pH 6.0 Spodoptera frugiperda
4.9
-
 4-nitrophenyl beta-D-xyloside mutant E190A, 30°C, pH 6.0 Spodoptera frugiperda
4.9
-
 4-nitrophenyl beta-D-xyloside mutant E194A, 30°C, pH 6.0 Spodoptera frugiperda
4.9
-
 4-nitrophenyl beta-D-galactoside mutant K201A, 30°C, pH 6.0 Spodoptera frugiperda
6.2
-
 4-methylumbeliferyl beta-D-glucoside mutant E190Q, 30°C, pH 6.0 Spodoptera frugiperda
6.2
-
 4-nitrophenyl beta-D-galactoside mutant K201F, 30°C, pH 6.0 Spodoptera frugiperda
6.3
-
 4-methylumbeliferyl beta-D-glucoside mutant E190A, 30°C, pH 6.0 Spodoptera frugiperda
8.2
-
 4-nitrophenyl beta-D-galactoside mutant E190A, 30°C, pH 6.0 Spodoptera frugiperda
9
-
 4-nitrophenyl beta-D-galactoside mutant E194A, 30°C, pH 6.0 Spodoptera frugiperda
9.9
-
 4-nitrophenyl beta-D-galactoside mutant E190Q, 30°C, pH 6.0 Spodoptera frugiperda

Organism

Organism UniProt Comment Textmining
Spodoptera frugiperda O61594
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbeliferyl beta-D-glucoside + H2O
-
 Spodoptera frugiperda 4-methylumbelliferol + D-glucose
-
 ?
4-nitrophenyl beta-D-fucoside + H2O
-
 Spodoptera frugiperda 4-nitrophenol + D-fucose
-
 ?
4-nitrophenyl beta-D-galactoside + H2O
-
 Spodoptera frugiperda 4-nitrophenol + D-galactose
-
 ?
4-nitrophenyl beta-D-glucoside + H2O
-
 Spodoptera frugiperda 4-nitrophenol + D-glucose
-
 ?
4-nitrophenyl beta-D-xyloside + H2O
-
 Spodoptera frugiperda 4-nitrophenol + D-xylose
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00188
-
 4-nitrophenyl beta-D-xyloside mutant E190A, 30°C, pH 6.0 Spodoptera frugiperda
0.0073
-
 4-nitrophenyl beta-D-xyloside mutant K201A, 30°C, pH 6.0 Spodoptera frugiperda
0.0127
-
 4-nitrophenyl beta-D-xyloside mutant E194A, 30°C, pH 6.0 Spodoptera frugiperda
0.02
-
 4-methylumbeliferyl beta-D-glucoside mutant E190A, 30°C, pH 6.0 Spodoptera frugiperda
0.0211
-
 4-nitrophenyl beta-D-xyloside mutant E190Q, 30°C, pH 6.0 Spodoptera frugiperda
0.0238
-
 4-nitrophenyl beta-D-xyloside mutant M453A, 30°C, pH 6.0 Spodoptera frugiperda
0.0302
-
 4-nitrophenyl beta-D-xyloside wild-type, 30°C, pH 6.0 Spodoptera frugiperda
0.032
-
 4-methylumbeliferyl beta-D-glucoside mutant K201A, 30°C, pH 6.0 Spodoptera frugiperda
0.058
-
 4-nitrophenyl beta-D-galactoside mutant K201A, 30°C, pH 6.0 Spodoptera frugiperda
0.072
-
 4-nitrophenyl beta-D-galactoside mutant E190A, 30°C, pH 6.0 Spodoptera frugiperda
0.089
-
 4-nitrophenyl beta-D-xyloside mutant K201F, 30°C, pH 6.0 Spodoptera frugiperda
0.114
-
 4-methylumbeliferyl beta-D-glucoside mutant E190Q, 30°C, pH 6.0 Spodoptera frugiperda
0.153
-
 4-nitrophenyl beta-D-galactoside mutant E190Q, 30°C, pH 6.0 Spodoptera frugiperda
0.174
-
 4-nitrophenyl beta-D-galactoside mutant M453A, 30°C, pH 6.0 Spodoptera frugiperda
0.189
-
 4-nitrophenyl beta-D-galactoside mutant E194A, 30°C, pH 6.0 Spodoptera frugiperda
0.231
-
 4-methylumbeliferyl beta-D-glucoside wild-type, 30°C, pH 6.0 Spodoptera frugiperda
0.2674
-
 4-methylumbeliferyl beta-D-glucoside mutant E194A, 30°C, pH 6.0 Spodoptera frugiperda
0.269
-
 4-nitrophenyl beta-D-galactoside wild-type, 30°C, pH 6.0 Spodoptera frugiperda
0.348
-
 4-nitrophenyl beta-D-fucoside mutant E190A, 30°C, pH 6.0 Spodoptera frugiperda
0.56
-
 4-nitrophenyl beta-D-glucoside mutant E194A, 30°C, pH 6.0 Spodoptera frugiperda
0.59
-
 4-nitrophenyl beta-D-glucoside mutant E190A, 30°C, pH 6.0 Spodoptera frugiperda
0.62
-
 4-methylumbeliferyl beta-D-glucoside mutant M453A, 30°C, pH 6.0 Spodoptera frugiperda
0.65
-
 4-nitrophenyl beta-D-glucoside mutant E190Q, 30°C, pH 6.0 Spodoptera frugiperda
0.66
-
 4-nitrophenyl beta-D-glucoside mutant K201A, 30°C, pH 6.0 Spodoptera frugiperda
0.668
-
 4-nitrophenyl beta-D-fucoside mutant K201F, 30°C, pH 6.0 Spodoptera frugiperda
0.98
-
 4-nitrophenyl beta-D-fucoside mutant E194A, 30°C, pH 6.0 Spodoptera frugiperda
1.32
-
 4-methylumbeliferyl beta-D-glucoside mutant K201F, 30°C, pH 6.0 Spodoptera frugiperda
1.52
-
 4-nitrophenyl beta-D-glucoside mutant M453A, 30°C, pH 6.0 Spodoptera frugiperda
2.26
-
 4-nitrophenyl beta-D-glucoside wild-type, 30°C, pH 6.0 Spodoptera frugiperda
2.43
-
 4-nitrophenyl beta-D-fucoside mutant K201A, 30°C, pH 6.0 Spodoptera frugiperda
4
-
 4-nitrophenyl beta-D-glucoside mutant K201F, 30°C, pH 6.0 Spodoptera frugiperda
4.03
-
 4-nitrophenyl beta-D-galactoside mutant K201F, 30°C, pH 6.0 Spodoptera frugiperda
4.15
-
 4-nitrophenyl beta-D-fucoside mutant M453A, 30°C, pH 6.0 Spodoptera frugiperda
7.14
-
 4-nitrophenyl beta-D-fucoside wild-type, 30°C, pH 6.0 Spodoptera frugiperda
8.9
-
 4-nitrophenyl beta-D-fucoside mutant K201F, 30°C, pH 6.0 Spodoptera frugiperda

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.04
-
 cellotriose mutant K201F, 30°C, pH 6.0 Spodoptera frugiperda
0.07
-
 cellotriose mutant E194A, 30°C, pH 6.0 Spodoptera frugiperda
0.09
-
 cellotriose mutant M453A, 30°C, pH 6.0 Spodoptera frugiperda
0.13
-
 nonyl beta-D-glucoside mutant K201F, 30°C, pH 6.0 Spodoptera frugiperda
0.14
-
 cellotetraose mutant E194A, 30°C, pH 6.0 Spodoptera frugiperda
0.2
-
 cellopentaose mutant E194A, 30°C, pH 6.0 Spodoptera frugiperda
0.2
-
 cellotetraose wild-type, 30°C, pH 6.0 Spodoptera frugiperda
0.21
-
 cellotetraose mutant M453A, 30°C, pH 6.0 Spodoptera frugiperda
0.22
-
 nonyl beta-D-glucoside wild-type, 30°C, pH 6.0 Spodoptera frugiperda
0.25
-
 cellotetraose mutant K201F, 30°C, pH 6.0 Spodoptera frugiperda
0.25
-
 cellopentaose wild-type, 30°C, pH 6.0 Spodoptera frugiperda
0.26
-
 cellopentaose mutant M453A, 30°C, pH 6.0 Spodoptera frugiperda
0.27
-
 cellotriose wild-type, 30°C, pH 6.0 Spodoptera frugiperda
0.3
-
 cellopentaose mutant K201F, 30°C, pH 6.0 Spodoptera frugiperda
0.41
-
 cellotriose mutant K201A, 30°C, pH 6.0 Spodoptera frugiperda
0.42
-
 octyl beta-D-glucoside mutant K201F, 30°C, pH 6.0 Spodoptera frugiperda
0.44
-
 octyl beta-D-glucoside wild-type, 30°C, pH 6.0 Spodoptera frugiperda
0.5
-
 nonyl beta-D-glucoside mutant M453A, 30°C, pH 6.0 Spodoptera frugiperda
0.54
-
 cellotetraose mutant K201A, 30°C, pH 6.0 Spodoptera frugiperda
0.7
-
 heptyl beta-D-glucoside wild-type, 30°C, pH 6.0 Spodoptera frugiperda
0.83
-
 nonyl beta-D-glucoside mutant E194A, 30°C, pH 6.0 Spodoptera frugiperda
1.12
-
 heptyl beta-D-glucoside mutant K201F, 30°C, pH 6.0 Spodoptera frugiperda
1.16
-
 nonyl beta-D-glucoside mutant K201A, 30°C, pH 6.0 Spodoptera frugiperda
1.2
-
 cellobiose mutant E194A, 30°C, pH 6.0 Spodoptera frugiperda
1.3
-
 octyl beta-D-glucoside mutant M453A, 30°C, pH 6.0 Spodoptera frugiperda
1.49
-
 octyl beta-D-glucoside mutant E194A, 30°C, pH 6.0 Spodoptera frugiperda
1.57
-
 hexyl beta-D-glucoside wild-type, 30°C, pH 6.0 Spodoptera frugiperda
2
-
 cellopentaose mutant E190A, 30°C, pH 6.0 Spodoptera frugiperda
2.3
-
 cellotriose mutant E190A, 30°C, pH 6.0 Spodoptera frugiperda
2.3
-
 cellopentaose mutant K201A, 30°C, pH 6.0 Spodoptera frugiperda
2.4
-
 cellotetraose mutant E190A, 30°C, pH 6.0 Spodoptera frugiperda
2.52
-
 octyl beta-D-glucoside mutant K201A, 30°C, pH 6.0 Spodoptera frugiperda
2.6
-
 heptyl beta-D-glucoside mutant M453A, 30°C, pH 6.0 Spodoptera frugiperda
2.9
-
 cellobiose wild-type, 30°C, pH 6.0 Spodoptera frugiperda
3.3
-
 hexyl beta-D-glucoside mutant K201F, 30°C, pH 6.0 Spodoptera frugiperda
3.6
-
 heptyl beta-D-glucoside mutant E194A, 30°C, pH 6.0 Spodoptera frugiperda
4.2
-
 heptyl beta-D-glucoside mutant K201A, 30°C, pH 6.0 Spodoptera frugiperda
5.1
-
 methyl beta-D-glucoside wild-type, 30°C, pH 6.0 Spodoptera frugiperda
5.2
-
 cellobiose mutant M453A, 30°C, pH 6.0 Spodoptera frugiperda
5.6
-
 hexyl beta-D-glucoside mutant M453A, 30°C, pH 6.0 Spodoptera frugiperda
5.7
-
 nonyl beta-D-glucoside mutant E190Q, 30°C, pH 6.0 Spodoptera frugiperda
5.9
-
 nonyl beta-D-glucoside mutant E190A, 30°C, pH 6.0 Spodoptera frugiperda
8
-
 methyl beta-D-glucoside mutant E190A, 30°C, pH 6.0 Spodoptera frugiperda
8
-
 hexyl beta-D-glucoside mutant E194A, 30°C, pH 6.0 Spodoptera frugiperda
8.6
-
 cellotriose mutant E190Q, 30°C, pH 6.0 Spodoptera frugiperda
10
-
 hexyl beta-D-glucoside mutant K201A, 30°C, pH 6.0 Spodoptera frugiperda
10
-
 methyl beta-D-glucoside mutant K201F, 30°C, pH 6.0 Spodoptera frugiperda
10.1
-
 methyl beta-D-glucoside mutant E194A, 30°C, pH 6.0 Spodoptera frugiperda
10.2
-
 methyl beta-D-glucoside mutant M453A, 30°C, pH 6.0 Spodoptera frugiperda
10.3
-
 octyl beta-D-glucoside mutant E190A, 30°C, pH 6.0 Spodoptera frugiperda
11
-
 octyl beta-D-glucoside mutant E190Q, 30°C, pH 6.0 Spodoptera frugiperda
12.1
-
 methyl beta-D-glucoside mutant E190Q, 30°C, pH 6.0 Spodoptera frugiperda
13.7
-
 cellobiose mutant E190Q, 30°C, pH 6.0 Spodoptera frugiperda
15.6
-
 methyl beta-D-glucoside mutant K201A, 30°C, pH 6.0 Spodoptera frugiperda
16.2
-
 cellopentaose mutant E190Q, 30°C, pH 6.0 Spodoptera frugiperda
17
-
 cellobiose mutant E190A, 30°C, pH 6.0 Spodoptera frugiperda
19.6
-
 cellotetraose mutant E190Q, 30°C, pH 6.0 Spodoptera frugiperda
21
-
 heptyl beta-D-glucoside mutant E190A, 30°C, pH 6.0 Spodoptera frugiperda
21.8
-
 heptyl beta-D-glucoside mutant E190Q, 30°C, pH 6.0 Spodoptera frugiperda
30
-
 phenyl beta-D-glucoside mutant E190A, 30°C, pH 6.0 Spodoptera frugiperda
30
-
 cellobiose mutant K201F, 30°C, pH 6.0 Spodoptera frugiperda
32
-
 hexyl beta-D-glucoside mutant E190A, 30°C, pH 6.0 Spodoptera frugiperda
36
-
 phenyl beta-D-glucoside mutant E194A, 30°C, pH 6.0 Spodoptera frugiperda
44.9
-
 hexyl beta-D-glucoside mutant E190Q, 30°C, pH 6.0 Spodoptera frugiperda
48
-
 phenyl beta-D-glucoside mutant K201F, 30°C, pH 6.0 Spodoptera frugiperda
49
-
 phenyl beta-D-glucoside wild-type, 30°C, pH 6.0 Spodoptera frugiperda
52
-
 phenyl beta-D-glucoside mutant M453A, 30°C, pH 6.0 Spodoptera frugiperda
74
-
 phenyl beta-D-glucoside mutant K201A, 30°C, pH 6.0 Spodoptera frugiperda
78
-
 phenyl beta-D-glucoside mutant E190Q, 30°C, pH 6.0 Spodoptera frugiperda
81
-
 cellobiose mutant K201A, 30°C, pH 6.0 Spodoptera frugiperda