Cloned (Comment) | Organism |
---|---|
gene celG encoded in the celEFG operon, construction of a cosmid library and cloning of the celEFG operon, genetic organizatin, DNA and amino acid sequence determination and analysis, phylogenetic tree, overexpression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) | Pectobacterium carotovorum |
Protein Variants | Comment | Organism |
---|---|---|
E172A | site-directed mutagenesis, inactive catalytic residue mutant | Pectobacterium carotovorum |
E370A | site-directed mutagenesis, inactive catalytic residue mutant | Pectobacterium carotovorum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Pectobacterium carotovorum | |
Co2+ | - |
Pectobacterium carotovorum | |
Cu2+ | - |
Pectobacterium carotovorum | |
Hg2+ | - |
Pectobacterium carotovorum | |
Mg2+ | - |
Pectobacterium carotovorum | |
Mn2+ | - |
Pectobacterium carotovorum | |
Zn2+ | - |
Pectobacterium carotovorum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
54000 | - |
x * 54000, CelG, SDS-PAGE | Pectobacterium carotovorum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cellobiose + H2O | Pectobacterium carotovorum | - |
2 beta-D-glucose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pectobacterium carotovorum | A1C3J9 | CelG, beta-glucosidase; subsp. carotovorum, strain LY34, gene celG | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli by nickel affinity chromatography and two steps of anion exchange chromatography | Pectobacterium carotovorum |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
celloheptaose + 6 H2O = 7 beta-D-glucose | the two glutamic acid residues Glu172 and Glu370 are essential for enzyme activity of CellG | Pectobacterium carotovorum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylumbelliferyl-beta-D-glucopyranoside + H2O | - |
Pectobacterium carotovorum | 4-methylumbelliferol + beta-D-glucose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Pectobacterium carotovorum | 4-nitrophenol + beta-D-glucose | - |
? | |
arbutin + H2O | - |
Pectobacterium carotovorum | ? | - |
? | |
cellobiose + H2O | - |
Pectobacterium carotovorum | 2 beta-D-glucose | - |
? | |
salicin + H2O | - |
Pectobacterium carotovorum | beta-D-glucose + 2-(hydroxymethyl)phenol | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 54000, CelG, SDS-PAGE | Pectobacterium carotovorum |
Synonyms | Comment | Organism |
---|---|---|
cellobiase | - |
Pectobacterium carotovorum |
More | the enzyme belongs to the glycosyl hydrolase family 1 | Pectobacterium carotovorum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Pectobacterium carotovorum |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 80 | - |
Pectobacterium carotovorum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Pectobacterium carotovorum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 9 | activity range of CelG | Pectobacterium carotovorum |